Expression of c-FLIPL and resistance to CD95-mediated apoptosis of monocyte-derived dendritic cells: inhibition by bisindolylmaleimide

F Willems, Z Amraoui, N Vanderheyde, V. Verhasselt, E Aksoy, C Scaffidi, ME Peter, PH Krammer, M Goldman

Research output: Contribution to journalArticle

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Abstract

To gain insight into the mechanisms controlling apoptosis of dendritic cells (DC), human monocyte-derived DC were analyzed for their expression of CD95 (Fas/Apo-1) and their response to CD95 ligation, Although DC expressed the CD95 molecule on their membrane, they did not undergo apoptosis on CD95 ligation unless sensitized by cycloheximide. In parallel, DC synthesized c-FLIPL, an inhibitor of the CD95-mediated death-signaling cascade. We also demonstrated that bisindolylmaleimide down-regulates c-FLIPL expression in DC and, in parallel, allows CD95-mediated apoptosis in these cells. In contrast, Bcl-2, Bcl-x(L),, and Bax levels were not affected by bisindolylmaleimide. We conclude that DC resist CD95-mediated apoptosis in association with c-FLIPL expression and that the immunosuppressive potential of bisindolylmaleimide previously observed at the T-cell level also involves facilitation of CD95-mediated DC apoptosis. (Blood, 2000;95: 3478-3482) (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)3478-3482
Number of pages5
JournalBlood
Volume95
Issue number11
Publication statusPublished - 1 Jun 2000

Cite this

Willems, F., Amraoui, Z., Vanderheyde, N., Verhasselt, V., Aksoy, E., Scaffidi, C., ... Goldman, M. (2000). Expression of c-FLIPL and resistance to CD95-mediated apoptosis of monocyte-derived dendritic cells: inhibition by bisindolylmaleimide. Blood, 95(11), 3478-3482.
Willems, F ; Amraoui, Z ; Vanderheyde, N ; Verhasselt, V. ; Aksoy, E ; Scaffidi, C ; Peter, ME ; Krammer, PH ; Goldman, M. / Expression of c-FLIPL and resistance to CD95-mediated apoptosis of monocyte-derived dendritic cells : inhibition by bisindolylmaleimide. In: Blood. 2000 ; Vol. 95, No. 11. pp. 3478-3482.
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abstract = "To gain insight into the mechanisms controlling apoptosis of dendritic cells (DC), human monocyte-derived DC were analyzed for their expression of CD95 (Fas/Apo-1) and their response to CD95 ligation, Although DC expressed the CD95 molecule on their membrane, they did not undergo apoptosis on CD95 ligation unless sensitized by cycloheximide. In parallel, DC synthesized c-FLIPL, an inhibitor of the CD95-mediated death-signaling cascade. We also demonstrated that bisindolylmaleimide down-regulates c-FLIPL expression in DC and, in parallel, allows CD95-mediated apoptosis in these cells. In contrast, Bcl-2, Bcl-x(L),, and Bax levels were not affected by bisindolylmaleimide. We conclude that DC resist CD95-mediated apoptosis in association with c-FLIPL expression and that the immunosuppressive potential of bisindolylmaleimide previously observed at the T-cell level also involves facilitation of CD95-mediated DC apoptosis. (Blood, 2000;95: 3478-3482) (C) 2000 by The American Society of Hematology.",
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Willems, F, Amraoui, Z, Vanderheyde, N, Verhasselt, V, Aksoy, E, Scaffidi, C, Peter, ME, Krammer, PH & Goldman, M 2000, 'Expression of c-FLIPL and resistance to CD95-mediated apoptosis of monocyte-derived dendritic cells: inhibition by bisindolylmaleimide' Blood, vol. 95, no. 11, pp. 3478-3482.

Expression of c-FLIPL and resistance to CD95-mediated apoptosis of monocyte-derived dendritic cells : inhibition by bisindolylmaleimide. / Willems, F; Amraoui, Z; Vanderheyde, N; Verhasselt, V.; Aksoy, E; Scaffidi, C; Peter, ME; Krammer, PH; Goldman, M.

In: Blood, Vol. 95, No. 11, 01.06.2000, p. 3478-3482.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of c-FLIPL and resistance to CD95-mediated apoptosis of monocyte-derived dendritic cells

T2 - inhibition by bisindolylmaleimide

AU - Willems, F

AU - Amraoui, Z

AU - Vanderheyde, N

AU - Verhasselt, V.

AU - Aksoy, E

AU - Scaffidi, C

AU - Peter, ME

AU - Krammer, PH

AU - Goldman, M

PY - 2000/6/1

Y1 - 2000/6/1

N2 - To gain insight into the mechanisms controlling apoptosis of dendritic cells (DC), human monocyte-derived DC were analyzed for their expression of CD95 (Fas/Apo-1) and their response to CD95 ligation, Although DC expressed the CD95 molecule on their membrane, they did not undergo apoptosis on CD95 ligation unless sensitized by cycloheximide. In parallel, DC synthesized c-FLIPL, an inhibitor of the CD95-mediated death-signaling cascade. We also demonstrated that bisindolylmaleimide down-regulates c-FLIPL expression in DC and, in parallel, allows CD95-mediated apoptosis in these cells. In contrast, Bcl-2, Bcl-x(L),, and Bax levels were not affected by bisindolylmaleimide. We conclude that DC resist CD95-mediated apoptosis in association with c-FLIPL expression and that the immunosuppressive potential of bisindolylmaleimide previously observed at the T-cell level also involves facilitation of CD95-mediated DC apoptosis. (Blood, 2000;95: 3478-3482) (C) 2000 by The American Society of Hematology.

AB - To gain insight into the mechanisms controlling apoptosis of dendritic cells (DC), human monocyte-derived DC were analyzed for their expression of CD95 (Fas/Apo-1) and their response to CD95 ligation, Although DC expressed the CD95 molecule on their membrane, they did not undergo apoptosis on CD95 ligation unless sensitized by cycloheximide. In parallel, DC synthesized c-FLIPL, an inhibitor of the CD95-mediated death-signaling cascade. We also demonstrated that bisindolylmaleimide down-regulates c-FLIPL expression in DC and, in parallel, allows CD95-mediated apoptosis in these cells. In contrast, Bcl-2, Bcl-x(L),, and Bax levels were not affected by bisindolylmaleimide. We conclude that DC resist CD95-mediated apoptosis in association with c-FLIPL expression and that the immunosuppressive potential of bisindolylmaleimide previously observed at the T-cell level also involves facilitation of CD95-mediated DC apoptosis. (Blood, 2000;95: 3478-3482) (C) 2000 by The American Society of Hematology.

KW - FAS-MEDIATED APOPTOSIS

KW - T-CELLS

KW - CD40 LIGATION

KW - HUMAN BLOOD

KW - SIGNALS

KW - ACTIVATION

KW - RECEPTOR

KW - NECROSIS

M3 - Article

VL - 95

SP - 3478

EP - 3482

JO - Blood

JF - Blood

SN - 0006-4971

IS - 11

ER -