Expression and localization of cyclo-oxygenase isoforms in non-small cell lung cancer

D.N. Watkins, J.L. Lenzo, M. Segal, M.J. Garlepp, Philip Thompson

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53 Citations (Scopus)


The beneficial effects of cyclo-oxgenase (COX) inhibitors in both colon cancer and adenomatous polyps suggest a role for the prostanoid pathway Tn epithelial malignancy. Although variable prostanoid synthesis in non-small cell lung cancer (NSCLC) has been demonstrated in freshly obtained tissue, COX messenger ribonucleic acid (mRNA) and protein localization in such tumours had not been investigated ex vivo.Thirty-four cases of primary NSCLC were examined for both constitutive (COX-I) and inducible COX (COX-2) by means of in situ hybridization and immunohistochemistry.COX-I mRNA expression was absent or below the level of detection via in situ hybridization. COX-I immunohistochemistry demonstrated uniform hint cytoplasmic staining in tumour cells and stromal inflammatory cells. Semiquantitative analysis of COX-2 expression in NSCLC demonstrated the highest levels of both mRNA and protein in adenocarcinoma cells (n=10, p
Original languageEnglish
Pages (from-to)412-418
JournalEuropean Respiratory Journal
Issue number2
Publication statusPublished - 1999


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