Abstract
Abiotic stresses are responsible for the massive loss of major grain crops all over the world. Many researchers in the world are trying to find the molecular pathways related to abiotic stress response in rice. Epigenetics is one of the ways to study plants' response to any stress. Polycomb groups of proteins are involved in the repression of many genes required at various developmental stages of a plant via chromatin-related changes like post-translational modification of histones, variation of nucleosome composition, and positioning of nucleosome by ATP-dependent chromatin remodeling complexes. The Polycomb group proteins function by forming larger complexes called Polycomb Repressive Complex which have been categorized as PRC2 and PRC1. The objective of the project was to examine the expression of 13 Polycomb genes in drought and salinity stress by real-time PCR. After the 1h of salt stress treatment, RING2, MCS2, FIE1 and, VRN1 were up-regulated and EV2 was down-regulated (1.5 or above fold-change) and after 24 h FIE2, LHP, RING2 and, VRN3 were up-regulated and VRN1 was down-regulated. Meanwhile, after 1 h of drought stress FIE2, RING2 and, VRN1 were up-regulated and MSI2, BM1, and VRN3 were down-regulated and after 24 h FIE2, RING2 and, VRN3 were up-regulated and MSI1 was down-regulated. Based on this study, the differential expression of these genes reveals their responsive nature toward salinity and drought stress and hence it becomes important to further analyze their role in abiotic stress tolerance in rice.
Original language | English |
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Qualification | Masters |
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Award date | 3 Jun 2019 |
Publication status | Unpublished - 3 Jun 2019 |