Exploring the combinatorial effects of epigenetic modifiers upon the mammalian genome using CRISPR-dCas9

Tessa Swain

doi:10.26182/tykn-af19

Exploring the combinatorial effects of epigenetic modifiers upon the mammalian genome using CRISPR-dCas9

Tessa Swain

UWA Centre for Medical Research (affiliated with the Harry Perkins Institute of Medical Research)

Research output: Thesis › Doctoral Thesis

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Abstract

The epigenome consists of chemical modifications to DNA and histone proteins that can regulate chromatin structure, DNA accessibility, and transcription. Given the complexity of epigenetic modifications, and the array of enzymes that regulate them, understanding the role of these modifications in transcriptional regulation and subsequently using them to control gene activity requires the exploration of their combinatorial activities. To this end, this thesis describes the expansion and characterisation of several novel epigenome editing tools that enable potent combinatorial recruitment and improved efficacy compared to current systems, laying the foundations for future research to probe the complex epigenomic landscape.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Lister, Ryan, Supervisor Pflueger, Christian, Supervisor Millar, Harvey, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	11 Dec 2020
DOIs	https://doi.org/10.26182/tykn-af19
Publication status	Unpublished - 2020

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10.26182/tykn-af19

THESIS - DOCTOR OF PHILOSOPHY - SWAIN, Tessa Alice Rose - 2020
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title = "Exploring the combinatorial effects of epigenetic modifiers upon the mammalian genome using CRISPR-dCas9",

abstract = "The epigenome consists of chemical modifications to DNA and histone proteins that can regulate chromatin structure, DNA accessibility, and transcription. Given the complexity of epigenetic modifications, and the array of enzymes that regulate them, understanding the role of these modifications in transcriptional regulation and subsequently using them to control gene activity requires the exploration of their combinatorial activities. To this end, this thesis describes the expansion and characterisation of several novel epigenome editing tools that enable potent combinatorial recruitment and improved efficacy compared to current systems, laying the foundations for future research to probe the complex epigenomic landscape.",

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N2 - The epigenome consists of chemical modifications to DNA and histone proteins that can regulate chromatin structure, DNA accessibility, and transcription. Given the complexity of epigenetic modifications, and the array of enzymes that regulate them, understanding the role of these modifications in transcriptional regulation and subsequently using them to control gene activity requires the exploration of their combinatorial activities. To this end, this thesis describes the expansion and characterisation of several novel epigenome editing tools that enable potent combinatorial recruitment and improved efficacy compared to current systems, laying the foundations for future research to probe the complex epigenomic landscape.

AB - The epigenome consists of chemical modifications to DNA and histone proteins that can regulate chromatin structure, DNA accessibility, and transcription. Given the complexity of epigenetic modifications, and the array of enzymes that regulate them, understanding the role of these modifications in transcriptional regulation and subsequently using them to control gene activity requires the exploration of their combinatorial activities. To this end, this thesis describes the expansion and characterisation of several novel epigenome editing tools that enable potent combinatorial recruitment and improved efficacy compared to current systems, laying the foundations for future research to probe the complex epigenomic landscape.

KW - combinatorial epigenetic

KW - CRISPR-dCas9

KW - DNA methylation

KW - Transcriptional regulation

KW - single cell RNA sequencing

KW - SunTag

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DO - 10.26182/tykn-af19

M3 - Doctoral Thesis

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Exploring the combinatorial effects of epigenetic modifiers upon the mammalian genome using CRISPR-dCas9

Abstract

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