Exploring the combinatorial effects of epigenetic modifiers upon the mammalian genome using CRISPR-dCas9

Tessa Swain

Research output: ThesisDoctoral Thesis

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Abstract

The epigenome consists of chemical modifications to DNA and histone proteins that can regulate chromatin structure, DNA accessibility, and transcription. Given the complexity of epigenetic modifications, and the array of enzymes that regulate them, understanding the role of these modifications in transcriptional regulation and subsequently using them to control gene activity requires the exploration of their combinatorial activities. To this end, this thesis describes the expansion and characterisation of several novel epigenome editing tools that enable potent combinatorial recruitment and improved efficacy compared to current systems, laying the foundations for future research to probe the complex epigenomic landscape.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Lister, Ryan, Supervisor
  • Pflueger, Christian, Supervisor
  • Millar, Harvey, Supervisor
Thesis sponsors
Award date11 Dec 2020
DOIs
Publication statusUnpublished - 2020

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