@article{e51ef6b3f2574992b6a931cb62301469,
title = "Exploring potential applications of a novel extracellular polymeric substance synthesizing bacterium (Bacillus licheniformis) isolated from gut contents of earthworm (Metaphire posthuma) in environmental remediation",
abstract = "The aim was to isolate, characterize, and explore potentials of gut bacteria from the earthworm (Metaphire posthuma) and imply these bacteria for remediation of Cu(II) and Zn(II). An extracellular polymeric substance (EPS) producing gut bacteria (Bacillus licheniformis strain KX657843) was isolated and identified based on 16S rRNA sequencing and phylogenetic analysis. The strain showed maximum tolerance of 8 and 6 mM for Cu(II) and Zn(II) respectively. It removed 34.5% of Cu(II) and 54.4% of Zn(II) at 25 mg L−1 after 72 and 96 h incubation respectively. The bacteria possessed a great potential to produce indole acetic acid (38.49 μg mL−1) at 5 mg mL−1l-tryptophan following 12 days incubation. The sterilized seeds of mung beans (Vigna radiata) displayed greater germination and growth under bacterium enriched condition. We observed that the bacterial strain phosphate solubilization ability with a maximum of 204.2 mg L−1 in absence of Cu(II) and Zn(II). Endowed with biosurfactant property the bacterium exhibited 24% emulsification index. The bacterium offered significant potential of plant growth promotion, Cu(II) and Zn(II) removal, and as such this study is the first report on EPS producing B. licheniformis KX657843 from earthworm which can be applied as powerful tool in remediation programs of Cu(II) and Zn(II) contaminated sites.",
keywords = "Biosurfactant, Gut bacteria, Heavy metal removal, Indole acetic acid, Phosphate solubilization, Vigna radiata",
author = "Biswas, {Jayanta Kumar} and Anurupa Banerjee and Rai, {Mahendra Kumar} and J{\"o}rg Rinklebe and Shaheen, {Sabry M.} and Sarkar, {Santosh Kumar} and Dash, {Madhab Chandra} and Anilava Kaviraj and Uwe Langer and Hocheol Song and Meththika Vithanage and Monojit Mondal and Niazi, {Nabeel Khan}",
note = "Funding Information: Acknowledgements The study was financially supported by Department of Science and Technology (DST), Government of India in the form of providing INSPIRE Fellowship (Code No.: 1F131101) to Anurupa Banerjee for carrying out research work under the supervision of Dr. Jayanta Kumar Biswas. The authors also duly acknowledge the infrastructural support provided by Department of Ecological Studies, University of Kalyani as well as the facility available under the DST-PURSE programme. Funding Information: The study was financially supported by Department of Science and Technology (DST), Government of India in the form of providing INSPIRE Fellowship (Code No.: 1F131101) to Anurupa Banerjee for carrying out research work under the supervision of Dr. Jayanta Kumar Biswas. The authors also duly acknowledge the infrastructural support provided by Department of Ecological Studies, University of Kalyani as well as the facility available under the DST-PURSE programme. There is no conflict of interest. Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media B.V., part of Springer Nature.",
year = "2018",
month = aug,
day = "1",
doi = "10.1007/s10532-018-9835-z",
language = "English",
volume = "29",
pages = "323--337",
journal = "Biodegradation",
issn = "0923-9820",
publisher = "Springer",
number = "4",
}