TY - JOUR
T1 - Experimental amniotic fluid infection in sheep : effects of Ureaplasma parvum serovars 3 and 6 on preterm or term fetal sheep
AU - Moss, T.J.
AU - Knox, C.L.
AU - Kallapur, S.G.
AU - Nitsos, Ilias
AU - Theodoropoulos, C.
AU - Newnham, John
AU - Ikegami, M.
AU - Jobe, Alan
PY - 2008
Y1 - 2008
N2 - Objective: The objective of the study was to determine the effects in late gestation of Ureaplasma parvum serovar 3 colonization and the effects, preterm, of U. parvum serovar 6.Study Design: Ewes received an intraamniotic (IA) injection of U. parvum serovar 6 (20 × 106 colony-forming units [cfu]; n = 9), U. parvum serovar 3 (20 × 103 cfu; n = 6), vehicle (n = 10), or saline (n = 4) on day 80 of pregnancy (d). The lambs were delivered at 125 d (U parvum serovar 6, n = 9; saline or media controls, n = 9) or 145 d (U parvum serovar 3, n = 6; media controls, n = 5) for assessment of inflammation and lung maturation.Results: IA ureaplasmas caused histologic chorioamnionitis but not preterm delivery. Fetal lung epithelium was colonized with ureaplasmas at both gestational ages, and pulmonary interleukin-8 levels had doubled in the ureaplasma-colonized animals, compared with the controls at 145 d. Surfactant levels in bronchoalveolar lavage fluid had increased 8-fold and 2.5-fold at 125 and 145 d, respectively, after ureaplasma injection. Conclusion: Fetal lung inflammation and altered development accompanies ureaplasma colonization, regardless of age at delivery.
AB - Objective: The objective of the study was to determine the effects in late gestation of Ureaplasma parvum serovar 3 colonization and the effects, preterm, of U. parvum serovar 6.Study Design: Ewes received an intraamniotic (IA) injection of U. parvum serovar 6 (20 × 106 colony-forming units [cfu]; n = 9), U. parvum serovar 3 (20 × 103 cfu; n = 6), vehicle (n = 10), or saline (n = 4) on day 80 of pregnancy (d). The lambs were delivered at 125 d (U parvum serovar 6, n = 9; saline or media controls, n = 9) or 145 d (U parvum serovar 3, n = 6; media controls, n = 5) for assessment of inflammation and lung maturation.Results: IA ureaplasmas caused histologic chorioamnionitis but not preterm delivery. Fetal lung epithelium was colonized with ureaplasmas at both gestational ages, and pulmonary interleukin-8 levels had doubled in the ureaplasma-colonized animals, compared with the controls at 145 d. Surfactant levels in bronchoalveolar lavage fluid had increased 8-fold and 2.5-fold at 125 and 145 d, respectively, after ureaplasma injection. Conclusion: Fetal lung inflammation and altered development accompanies ureaplasma colonization, regardless of age at delivery.
U2 - 10.1016/j.ajog.2007.06.065
DO - 10.1016/j.ajog.2007.06.065
M3 - Article
SN - 0002-9378
VL - 198
SP - 122.e1-122.e8
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
IS - 1
ER -