TY - JOUR
T1 - Ex vivo and in vitro assessment of anti-inflammatory activity of seed β-conglutin proteins from Lupinus angustifolius
AU - Lima-Cabello, Elena
AU - Morales-Santana, Sonia
AU - Foley, Rhonda C.
AU - Melser, Su
AU - Alché, Victor
AU - Siddique, Kadambot H.M.
AU - Singh, Karam B.
AU - Alché, Juan D.
AU - Jimenez-Lopez, Jose C.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The development of functional food ingredients using legume seed proteins has potential for nutraceutical use. We purified recombinant β-conglutin proteins (rβ1 to rβ4, and rβ6) from narrow-leafed lupin using affinity–chromatography, and evaluated their anti-inflammatory activity using ex vivo and in vitro systems. rβ1, rβ3, and rβ6 produced lower levels of pro-inflammatory mediators such as nitric oxide (about –34.0–fold in all cases), iNOS mRNA (−7.15, −7.97, −7.41–fold), interleukin 1β (−12.05, −11.64, −12.16–fold), chemokine CCL5 (−16.0, −18.0, −19.0–fold), and cytokines including TNF-α INF-γ IL-1β IL-2, IL-6, IL-8, and IL-12 (β1: −28, −100, −8, −2, −49, −45, −127–fold; β3: −22, −400, −9, −3, −33, −10, −2.54–fold; β6: −72, −122, −11, −3, −2000, −13, −338–fold). These results suggest that the β1, β3, and β6 conglutins have potential as functional food components in nutraceuticals and that can provide alternative therapies for the prevention and treatment of inflammatory-related diseases.
AB - The development of functional food ingredients using legume seed proteins has potential for nutraceutical use. We purified recombinant β-conglutin proteins (rβ1 to rβ4, and rβ6) from narrow-leafed lupin using affinity–chromatography, and evaluated their anti-inflammatory activity using ex vivo and in vitro systems. rβ1, rβ3, and rβ6 produced lower levels of pro-inflammatory mediators such as nitric oxide (about –34.0–fold in all cases), iNOS mRNA (−7.15, −7.97, −7.41–fold), interleukin 1β (−12.05, −11.64, −12.16–fold), chemokine CCL5 (−16.0, −18.0, −19.0–fold), and cytokines including TNF-α INF-γ IL-1β IL-2, IL-6, IL-8, and IL-12 (β1: −28, −100, −8, −2, −49, −45, −127–fold; β3: −22, −400, −9, −3, −33, −10, −2.54–fold; β6: −72, −122, −11, −3, −2000, −13, −338–fold). These results suggest that the β1, β3, and β6 conglutins have potential as functional food components in nutraceuticals and that can provide alternative therapies for the prevention and treatment of inflammatory-related diseases.
KW - Anti-inflammatory proteins
KW - chemokine CCL5
KW - IL-1β
KW - iNOS
KW - Lupinus angustifolius
KW - Vicilin
KW - β-conglutins
UR - http://www.scopus.com/inward/record.url?scp=85036555303&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2017.11.040
DO - 10.1016/j.jff.2017.11.040
M3 - Article
AN - SCOPUS:85036555303
SN - 1756-4646
VL - 40
SP - 510
EP - 519
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -