Ex situ germplasm preservation and plant regeneration of a threatened terrestrial orchid, Caladenia huegelii, through micropropagation and cryopreservation

Betty M. Bustam, Kingsley Dixon, Eric Bunn

    Research output: Chapter in Book/Conference paperConference paperpeer-review

    6 Citations (Scopus)

    Abstract

    © 2013 CSIRO.Caladenia huegelii Rchb.f. is a threatened obligate mycotrophic orchid species with limited distribution in its remaining habitat, hence, this study to develop ex situ conservation protocols. An asymbiotic seed germination protocol was used to generate primary protocorms that were then used to proliferate secondary (adventitious protocorms). Both primary and secondary protocorms were utilised for development of a cryopreservation protocol. An average 94% germination of C. huegelii seeds occurred on MS medium with 5% (v/v) coconut water) and 43% primary protocorms formed secondary (adventitious) protocorms) on MS (- CW)+5&M ?-naphthaleneacetic acid+2&M BAP. Incubation at a constant 20±0.5°C after cryopreservation significantly increased regeneration of both primary and secondary protocorms at 79 and 45%, respectively, compared with 46 and 19% at a higher, more variable standard culture temperature range (22-26°C). Seedlings were successfully regenerated from both cryopreserved primary and secondary protocorms, but only plantlets derived from primary protocorms were investigated for transfer to soil, where 88% plantlets survived, with sustained growth (average leaf length increase of 4.1cm) after 12 weeks. This study demonstrates that plantlets derived from cryopreserved asymbiotically-produced protocorms can be established in soil and provide a feasible option to facilitate conservation and development of restoration protocols for endangered terrestrial orchids.
    Original languageEnglish
    Title of host publicationAustralian Journal of Botany
    EditorsM Bryne, D Williams
    PublisherCSIRO Publishing
    Pages659-663
    Number of pages5
    Volume64
    Edition8
    ISBN (Print)00671924
    DOIs
    Publication statusPublished - 2016
    Event2015 Ecological Society of Australia Conference - Adelaide, Australia
    Duration: 29 Nov 20153 Dec 2015

    Publication series

    NameAustralian Journal of Botany
    ISSN (Print)0067-1924

    Conference

    Conference2015 Ecological Society of Australia Conference
    Country/TerritoryAustralia
    CityAdelaide
    Period29/11/153/12/15

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