Evolving identification of blood cells associated with clinically isolated syndrome: Importance of time since clinical presentation and diagnostic MRI

Stephanie Trend, Anderson P. Jones, Sian Geldenhuys, Scott N. Byrne, Marzena J. Fabis-Pedrini, David Nolan, David R. Booth, William M. Carroll, Robyn M. Lucas, Allan G. Kermode, Prue H. Hart

Research output: Contribution to journalArticle

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Abstract

It is not clear how the profile of immune cells in peripheral blood differs between patients with clinically isolated syndrome (CIS) and healthy controls (HC). This study aimed to identify a CIS peripheral blood signature that may provide clues for potential immunomodulatory approaches early in disease. Peripheral blood mononuclear cells (PBMCs) were collected from 18 people with CIS, 19 HC and 13 individuals with other demyelinating conditions (ODC) including multiple sclerosis (MS). Individuals with CIS separated into two groups, namely those with early (≤14 days post-diagnostic magnetic resonance imaging (MRI); n = 6) and late (≥27 days; n = 12) blood sampling. Transitional B cells were increased in the blood of CIS patients independently of when blood was taken. However, there were two time-dependent effects found in the late CIS group relative to HC, including decreased CD56bright NK cells, which correlated significantly with time since MRI, and increased CD141+ myeloid dendritic cell (mDC2) frequencies. Higher CD1c+ B cells and lower non-classical monocyte frequencies were characteristic of more recent demyelinating disease activity (ODC and early CIS). Analysing cell populations by time since symptoms (subjective) and diagnostic MRI (objective) may contribute to understanding CIS.

Original languageEnglish
Article number1277
JournalInternational Journal of Molecular Sciences
Volume18
Issue number6
DOIs
Publication statusPublished - 15 Jun 2017

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blood cells
Magnetic resonance
magnetic resonance
Blood Cells
Blood
Cells
Magnetic Resonance Imaging
Imaging techniques
blood
cells
monocytes
B-Lymphoid Precursor Cells
Demyelinating Diseases
Myeloid Cells
Natural Killer Cells
Dendritic Cells
Sampling
Multiple Sclerosis
Monocytes
B-Lymphocytes

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Trend, Stephanie ; Jones, Anderson P. ; Geldenhuys, Sian ; Byrne, Scott N. ; Fabis-Pedrini, Marzena J. ; Nolan, David ; Booth, David R. ; Carroll, William M. ; Lucas, Robyn M. ; Kermode, Allan G. ; Hart, Prue H. / Evolving identification of blood cells associated with clinically isolated syndrome : Importance of time since clinical presentation and diagnostic MRI. In: International Journal of Molecular Sciences. 2017 ; Vol. 18, No. 6.
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abstract = "It is not clear how the profile of immune cells in peripheral blood differs between patients with clinically isolated syndrome (CIS) and healthy controls (HC). This study aimed to identify a CIS peripheral blood signature that may provide clues for potential immunomodulatory approaches early in disease. Peripheral blood mononuclear cells (PBMCs) were collected from 18 people with CIS, 19 HC and 13 individuals with other demyelinating conditions (ODC) including multiple sclerosis (MS). Individuals with CIS separated into two groups, namely those with early (≤14 days post-diagnostic magnetic resonance imaging (MRI); n = 6) and late (≥27 days; n = 12) blood sampling. Transitional B cells were increased in the blood of CIS patients independently of when blood was taken. However, there were two time-dependent effects found in the late CIS group relative to HC, including decreased CD56bright NK cells, which correlated significantly with time since MRI, and increased CD141+ myeloid dendritic cell (mDC2) frequencies. Higher CD1c+ B cells and lower non-classical monocyte frequencies were characteristic of more recent demyelinating disease activity (ODC and early CIS). Analysing cell populations by time since symptoms (subjective) and diagnostic MRI (objective) may contribute to understanding CIS.",
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Evolving identification of blood cells associated with clinically isolated syndrome : Importance of time since clinical presentation and diagnostic MRI. / Trend, Stephanie; Jones, Anderson P.; Geldenhuys, Sian; Byrne, Scott N.; Fabis-Pedrini, Marzena J.; Nolan, David; Booth, David R.; Carroll, William M.; Lucas, Robyn M.; Kermode, Allan G.; Hart, Prue H.

In: International Journal of Molecular Sciences, Vol. 18, No. 6, 1277, 15.06.2017.

Research output: Contribution to journalArticle

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T1 - Evolving identification of blood cells associated with clinically isolated syndrome

T2 - Importance of time since clinical presentation and diagnostic MRI

AU - Trend, Stephanie

AU - Jones, Anderson P.

AU - Geldenhuys, Sian

AU - Byrne, Scott N.

AU - Fabis-Pedrini, Marzena J.

AU - Nolan, David

AU - Booth, David R.

AU - Carroll, William M.

AU - Lucas, Robyn M.

AU - Kermode, Allan G.

AU - Hart, Prue H.

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Y1 - 2017/6/15

N2 - It is not clear how the profile of immune cells in peripheral blood differs between patients with clinically isolated syndrome (CIS) and healthy controls (HC). This study aimed to identify a CIS peripheral blood signature that may provide clues for potential immunomodulatory approaches early in disease. Peripheral blood mononuclear cells (PBMCs) were collected from 18 people with CIS, 19 HC and 13 individuals with other demyelinating conditions (ODC) including multiple sclerosis (MS). Individuals with CIS separated into two groups, namely those with early (≤14 days post-diagnostic magnetic resonance imaging (MRI); n = 6) and late (≥27 days; n = 12) blood sampling. Transitional B cells were increased in the blood of CIS patients independently of when blood was taken. However, there were two time-dependent effects found in the late CIS group relative to HC, including decreased CD56bright NK cells, which correlated significantly with time since MRI, and increased CD141+ myeloid dendritic cell (mDC2) frequencies. Higher CD1c+ B cells and lower non-classical monocyte frequencies were characteristic of more recent demyelinating disease activity (ODC and early CIS). Analysing cell populations by time since symptoms (subjective) and diagnostic MRI (objective) may contribute to understanding CIS.

AB - It is not clear how the profile of immune cells in peripheral blood differs between patients with clinically isolated syndrome (CIS) and healthy controls (HC). This study aimed to identify a CIS peripheral blood signature that may provide clues for potential immunomodulatory approaches early in disease. Peripheral blood mononuclear cells (PBMCs) were collected from 18 people with CIS, 19 HC and 13 individuals with other demyelinating conditions (ODC) including multiple sclerosis (MS). Individuals with CIS separated into two groups, namely those with early (≤14 days post-diagnostic magnetic resonance imaging (MRI); n = 6) and late (≥27 days; n = 12) blood sampling. Transitional B cells were increased in the blood of CIS patients independently of when blood was taken. However, there were two time-dependent effects found in the late CIS group relative to HC, including decreased CD56bright NK cells, which correlated significantly with time since MRI, and increased CD141+ myeloid dendritic cell (mDC2) frequencies. Higher CD1c+ B cells and lower non-classical monocyte frequencies were characteristic of more recent demyelinating disease activity (ODC and early CIS). Analysing cell populations by time since symptoms (subjective) and diagnostic MRI (objective) may contribute to understanding CIS.

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