Evaluating the diversity and composition of bacterial communities associated with Vachellia pachyceras - the only existing native tree species in the Kuwait desert

Majda K. Suleiman, Kingsley Dixon, Lucy Commander, Paul Nevill, Ali M. Quoreshi, Narayana R. Bhat, Anitha J. Manuvel, Mini T. Sivadasan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We investigated the diversity and composition of bacterial communities in rhizospheric and non-rhizospheric bulk soils as well as root nodule bacterial communities of Vachellia pachyceras-the only native tree species existing in the Kuwait desert. Community fingerprinting comparisons and 16S rDNA sequence identifications were used for characterization of the bacterial population using specific primers. The bacterial characterization of soil samples revealed four major phyla: Acidobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. In situ (desert) samples of both rhizospheric and non-rhizospheric bulk soil were dominated by the bacterial phyla Firmicutes and Bacteroidetes, whereas the phylum Betaproteobacteria was present only in non-rhizospheric bulk soil. Ex situ (nursery growing condition) V. pachyceras resulted in restricted bacterial communities dominated by members of a single phylum, Bacteroidetes. Results indicated that the soil organic matter and rhizospheric environments might drive the bacterial community. Despite harsh climatic conditions, data demonstrated that V. pachyceras roots harbor endophytic bacterial populations. Our findings on bacterial community composition and structure have major significance for evaluating how Kuwait's extreme climatic conditions affect bacterial communities. The baseline data obtained in this study will be useful and assist in formulating strategies in ecological restoration programs, including the application of inoculation technologies.

Original languageEnglish
Pages (from-to)235-251
Number of pages17
JournalCanadian Journal of Microbiology
Volume65
Issue number3
DOIs
Publication statusPublished - Mar 2019

Cite this

Suleiman, Majda K. ; Dixon, Kingsley ; Commander, Lucy ; Nevill, Paul ; Quoreshi, Ali M. ; Bhat, Narayana R. ; Manuvel, Anitha J. ; Sivadasan, Mini T. / Evaluating the diversity and composition of bacterial communities associated with Vachellia pachyceras - the only existing native tree species in the Kuwait desert. In: Canadian Journal of Microbiology. 2019 ; Vol. 65, No. 3. pp. 235-251.
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abstract = "We investigated the diversity and composition of bacterial communities in rhizospheric and non-rhizospheric bulk soils as well as root nodule bacterial communities of Vachellia pachyceras-the only native tree species existing in the Kuwait desert. Community fingerprinting comparisons and 16S rDNA sequence identifications were used for characterization of the bacterial population using specific primers. The bacterial characterization of soil samples revealed four major phyla: Acidobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. In situ (desert) samples of both rhizospheric and non-rhizospheric bulk soil were dominated by the bacterial phyla Firmicutes and Bacteroidetes, whereas the phylum Betaproteobacteria was present only in non-rhizospheric bulk soil. Ex situ (nursery growing condition) V. pachyceras resulted in restricted bacterial communities dominated by members of a single phylum, Bacteroidetes. Results indicated that the soil organic matter and rhizospheric environments might drive the bacterial community. Despite harsh climatic conditions, data demonstrated that V. pachyceras roots harbor endophytic bacterial populations. Our findings on bacterial community composition and structure have major significance for evaluating how Kuwait's extreme climatic conditions affect bacterial communities. The baseline data obtained in this study will be useful and assist in formulating strategies in ecological restoration programs, including the application of inoculation technologies.",
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Evaluating the diversity and composition of bacterial communities associated with Vachellia pachyceras - the only existing native tree species in the Kuwait desert. / Suleiman, Majda K.; Dixon, Kingsley; Commander, Lucy; Nevill, Paul; Quoreshi, Ali M.; Bhat, Narayana R.; Manuvel, Anitha J.; Sivadasan, Mini T.

In: Canadian Journal of Microbiology, Vol. 65, No. 3, 03.2019, p. 235-251.

Research output: Contribution to journalArticle

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AU - Suleiman, Majda K.

AU - Dixon, Kingsley

AU - Commander, Lucy

AU - Nevill, Paul

AU - Quoreshi, Ali M.

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AU - Sivadasan, Mini T.

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AB - We investigated the diversity and composition of bacterial communities in rhizospheric and non-rhizospheric bulk soils as well as root nodule bacterial communities of Vachellia pachyceras-the only native tree species existing in the Kuwait desert. Community fingerprinting comparisons and 16S rDNA sequence identifications were used for characterization of the bacterial population using specific primers. The bacterial characterization of soil samples revealed four major phyla: Acidobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. In situ (desert) samples of both rhizospheric and non-rhizospheric bulk soil were dominated by the bacterial phyla Firmicutes and Bacteroidetes, whereas the phylum Betaproteobacteria was present only in non-rhizospheric bulk soil. Ex situ (nursery growing condition) V. pachyceras resulted in restricted bacterial communities dominated by members of a single phylum, Bacteroidetes. Results indicated that the soil organic matter and rhizospheric environments might drive the bacterial community. Despite harsh climatic conditions, data demonstrated that V. pachyceras roots harbor endophytic bacterial populations. Our findings on bacterial community composition and structure have major significance for evaluating how Kuwait's extreme climatic conditions affect bacterial communities. The baseline data obtained in this study will be useful and assist in formulating strategies in ecological restoration programs, including the application of inoculation technologies.

KW - bacterial communities

KW - Kuwait desert

KW - Vachellia pachyceras

KW - molecular identification

KW - PCR cloning

KW - rhizospheric and non-rhizospheric bulk soils

KW - GRADIENT GEL-ELECTROPHORESIS

KW - SP-NOV.

KW - MICROBIAL COMMUNITIES

KW - PHYLOGENETIC ANALYSIS

KW - ACACIA-SENEGAL

KW - ATACAMA DESERT

KW - LOESS PLATEAU

KW - SOIL

KW - RHIZOBIUM

KW - RHIZOSPHERE

U2 - 10.1139/cjm-2018-0421

DO - 10.1139/cjm-2018-0421

M3 - Article

VL - 65

SP - 235

EP - 251

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

SN - 0008-4166

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ER -