Numerous stripe rust resistance genes have been identified from wheat, and new virulent races of Puccinia striiformis f. sp. tritici have also emerged in recent years. Deployment of diverse combinations of resistance genes is an efficient way to combat virulent evolution of strip rust pathogen. In this study, publically available molecular markers were used to identify the distribution of 36 Yr genes in 672 wheat accessions. The effectiveness of Yr genes individually and in combinations was also evaluated in field conditions. The result showed effective resistance of some recently applied genes, such as Yr15 and Yr65. It also showed the lost efficacy of some once widely used genes, such as Yr9 and Yr10. Moreover, significant additive effects were observed in some gene combinations, such as Yr9 + Yr18 and Yr30 + Yr46. Proper deploying of Yr genes and utilizing the positive interactions will be helpful for durable resistance breeding in wheat.