Estrogen Receptor Status by Immunohistochemistry Is Superior to the Ligand-Binding Assay for Predicting Response to Adjuvant Endocrine Therapy in Breast Cancer

Jennet Harvey, G.M. Clark, C.K. Osborne, D.C. Allred

    Research output: Contribution to journalArticlepeer-review

    1839 Citations (Scopus)

    Abstract

    Purpose: Immunohistochemistry (IHC) is a newer technique for assessing the estrogen receptor (ER) status of breast cancers, with the potential to overcome many of the shortcomings associated with the traditional ligand-binding assay (LBA), The purpose of this study was to evaluate the ability of ER status determination by IHC, compared with LBA, to predict clinical outcome-especially response to adjuvant endocrine therapy-in a large number of patients with long-term clinical follow-up.Patients and Methods: ER status was evaluated in 1,982 primary breast cancers by IHC on formalin-fixed paraffin-embedded tissue sections, using antibody 6F11 and standard methodology, Slides were scored on a scale representing the estimated proportion and intensity of positive-staining tumor cells (range, 0 to 8), Results were compared with ER values obtained by the LBA in the same tumors and to clinical outcome.Results: An IHC score of greater than 2 (corresponding to as few as 1% to 10% weakly positive cells) was used to define ER positivity on the basis of a univariate cut-point analysis of all possible scores and disease-free survival (DFS) in patients receiving any adjuvant endocrine therapy. Using this definition, 71% of all tumors were determined to be ER-positive by IHC, and the level of agreement with the LBA was 86%, In multivariate analyses of patients receiving adjuvant endocrine therapy alone, ER status determined by IHC was better than that determined by the LBA at predicting improved DFS (hazard ratios/P = 0.474/.0008 and 0.707/.3214, respectively) and equivalent at predicting overall survival (0.379/.0001 and 0.381/.0003, respectively).Conclusion: IHC is superior to the LBA for assessing ER status in primary breast cancer because it is easier, safer, and less expensive, and has an equivalent or better ability to predict response to adjuvant endocrine therapy. J Clin Oncol 17:1474-1481. (C) 1999 by American Society of Clinical Oncology.
    Original languageEnglish
    Pages (from-to)1474-1481
    JournalJournal of Clinical Oncology
    Volume17
    Issue number5 (May)
    Publication statusPublished - 1999

    Fingerprint

    Dive into the research topics of 'Estrogen Receptor Status by Immunohistochemistry Is Superior to the Ligand-Binding Assay for Predicting Response to Adjuvant Endocrine Therapy in Breast Cancer'. Together they form a unique fingerprint.

    Cite this