Establishment of a CRISPR/CasRx system to modify Turnip mosaic virus (TuMV) resistance in Brassica napus

Linh Ton

doi:10.26182/1z2s-ax86

Establishment of a CRISPR/CasRx system to modify Turnip mosaic virus (TuMV) resistance in Brassica napus

Linh Ton

School of Biological Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

Virus resistance breeding in canola (Brassica napus) has fallen behind the occurrence of resistance-breaking strains of RNA viruses, such as turnip mosaic virus (TuMV), presenting an increasing canola yield loss threat under climate scenarios. This thesis presents initial results for improving TuMV resistance for canola, including the establishment of a glasshouse system for TuMV resistance evaluation and regeneration of genetically transformed plants. The TuMV inoculation system was successfully implemented on micro-propagated seedlings of Cobbler cultivar, and CasRx-transgenic plants were obtained from the regeneration protocol, indicating the potential of using CRISPR/CasRxto interfere with RNA virus infection in this crop.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Batley, Jacqueline, Supervisor Jones, Roger, Supervisor Bayer, Philipp, Supervisor
Award date	21 Aug 2024
DOIs	https://doi.org/10.26182/1z2s-ax86
Publication status	Unpublished - 2024

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10.26182/1z2s-ax86

THESIS - DOCTOR OF PHILOSOPHY - TON Bao Linh - 2024_
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Cite this

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title = "Establishment of a CRISPR/CasRx system to modify Turnip mosaic virus (TuMV) resistance in Brassica napus",

abstract = "Virus resistance breeding in canola (Brassica napus) has fallen behind the occurrence of resistance-breaking strains of RNA viruses, such as turnip mosaic virus (TuMV), presenting an increasing canola yield loss threat under climate scenarios. This thesis presents initial results for improving TuMV resistance for canola, including the establishment of a glasshouse system for TuMV resistance evaluation and regeneration of genetically transformed plants. The TuMV inoculation system was successfully implemented on micro-propagated seedlings of Cobbler cultivar, and CasRx-transgenic plants were obtained from the regeneration protocol, indicating the potential of using CRISPR/CasRxto interfere with RNA virus infection in this crop.",

keywords = "Brassica napus, RNA viruses, turnip mosaic virus, CRISPR/CasRx, TuMV resistance, transgenic",

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language = "English",

school = "The University of Western Australia",

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N2 - Virus resistance breeding in canola (Brassica napus) has fallen behind the occurrence of resistance-breaking strains of RNA viruses, such as turnip mosaic virus (TuMV), presenting an increasing canola yield loss threat under climate scenarios. This thesis presents initial results for improving TuMV resistance for canola, including the establishment of a glasshouse system for TuMV resistance evaluation and regeneration of genetically transformed plants. The TuMV inoculation system was successfully implemented on micro-propagated seedlings of Cobbler cultivar, and CasRx-transgenic plants were obtained from the regeneration protocol, indicating the potential of using CRISPR/CasRxto interfere with RNA virus infection in this crop.

AB - Virus resistance breeding in canola (Brassica napus) has fallen behind the occurrence of resistance-breaking strains of RNA viruses, such as turnip mosaic virus (TuMV), presenting an increasing canola yield loss threat under climate scenarios. This thesis presents initial results for improving TuMV resistance for canola, including the establishment of a glasshouse system for TuMV resistance evaluation and regeneration of genetically transformed plants. The TuMV inoculation system was successfully implemented on micro-propagated seedlings of Cobbler cultivar, and CasRx-transgenic plants were obtained from the regeneration protocol, indicating the potential of using CRISPR/CasRxto interfere with RNA virus infection in this crop.

KW - Brassica napus

KW - RNA viruses

KW - turnip mosaic virus

KW - CRISPR/CasRx

KW - TuMV resistance

KW - transgenic

U2 - 10.26182/1z2s-ax86

DO - 10.26182/1z2s-ax86

M3 - Doctoral Thesis

ER -

Establishment of a CRISPR/CasRx system to modify Turnip mosaic virus (TuMV) resistance in Brassica napus

Abstract

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