Erfassung der Internalisierung von GPCRs mit CRISPR/Cas9

Andrea Krumm; Carl White; Kevin Pfleger

doi:10.1007/s12268-018-0975-9

Erfassung der Internalisierung von GPCRs mit CRISPR/Cas9

Translated title of the contribution: Detection of the internalization of GPCRs with CRISPR/Cas9

Andrea Krumm, Carl White, Kevin Pfleger

Research output: Contribution to journal › Article › peer-review

Abstract

Receptors are prevalent drug targets as they translate signals from outside the cell to cellular reactions. In drug finding and characterization it is not only required to measure ligand binding, but just as well subsequent regulatory steps including desensitizing protein interactions. Here, we show how CRISPR/Cas9 allowed endogenous expression of a G-protein coupled receptor (GPCR) and how that was used to study protein inter -actions and internalization of a cancer progressing receptor.

Translated title of the contribution	Detection of the internalization of GPCRs with CRISPR/Cas9
Original language	German
Pages (from-to)	616-617
Number of pages	2
Journal	BioSpektrum
Volume	24
Issue number	6
DOIs	https://doi.org/10.1007/s12268-018-0975-9
Publication status	Published - 1 Oct 2018

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10.1007/s12268-018-0975-9

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abstract = "Receptors are prevalent drug targets as they translate signals from outside the cell to cellular reactions. In drug finding and characterization it is not only required to measure ligand binding, but just as well subsequent regulatory steps including desensitizing protein interactions. Here, we show how CRISPR/Cas9 allowed endogenous expression of a G-protein coupled receptor (GPCR) and how that was used to study protein inter -actions and internalization of a cancer progressing receptor.",

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Erfassung der Internalisierung von GPCRs mit CRISPR/Cas9

Abstract

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