Epigenetic Modulators Enhance Constitutive and Liver-Specific Reporter Expression in Murine Liver Progenitor Cell Lines

Luke Diepeveen, Michel Watson, Sarah Mcspadden, R.P. Strauss, Bernard Callus, George Yeoh

Research output: Contribution to journalArticle

2 Citations (Scopus)
246 Downloads (Pure)

Abstract

© Copyright 2015, Mary Ann Liebert, Inc. 2015. Stem cells expressing reporter constructs are extremely useful for their tracking in vivo or for determining cell lineage fate in vivo and in vitro. We generated liver progenitor cell (LPC) lines from actin-EGFP and TAT-GRE-lacZ mice. LPCs from the actin-EGFP mouse facilitate cell tracing following transplant as the reporter is constitutively expressed. LPCs from the TAT-GRE-lacZ mouse express β-galactosidase under the control of the tyrosine aminotransferase (TAT) promoter and are only active in mature hepatocytes. We found that the utility of such LPC lines becomes severely limited by downregulation of transgene expression following extended culture. We show that epigenetic mechanisms are responsible for suppressing expression of both transgenes. Enhancement of transgene expression in both LPC lines was achieved by treating the cell lines with either the histone acetylating agent sodium butyrate or the DNA demethylating agent 5-azacytidine.
Original languageEnglish
Pages (from-to)1080-1087
Number of pages8
JournalTissue Engineering - Part C: Methods
Volume21
Issue number10
Early online date10 Jul 2015
DOIs
Publication statusPublished - 1 Oct 2015

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