Ephrin-A2 affects wound healing and scarring in a murine model of excisional injury

Research output: Contribution to journalArticle

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Abstract

Ephrin ligand/Eph receptor signaling is important in both tissue development and homeostasis. There is increasing evidence that Ephrin/Eph signaling is important in the skin, involved in hair follicle cycling, epidermal differentiation, cutaneous innervation and skin cancer. However, there is currently limited information on the role of Ephrin/Eph signaling in cutaneous wound healing. Here we report the effects of the Ephrin-A2 and A5 ligands on wound healing. Using Ephrin-A2-/-, Ephrin-A5-/- and Ephrin-A2A5-/- transgenic mice, in vitro wound healing assays were conducted using isolated keratinocytes and fibroblasts. Ephrin-A2-/-, Ephrin-A2A5-/- and wild type mice with excisional wounds were used to analyze the impact of these ligands on wound closure, scar outcome, collagen orientation and re-innervation in vivo. The absence of the Ephrin-A2 and A5 ligands did not have any effect on dermal fibroblast proliferation or on fibroblast or keratinocyte migration. The loss of Ephrin-A2 and A5 ligands did not impact on the rate of wound closure or re-innervation after injury. However, changes in the gross morphology of the healed scar and in collagen histology of the scar dermis were observed in transgenic mice. Therefore Ephrin-A2 and A5 ligands may play an important role in final scar appearance associated with collagen deposition and structure.

Original languageEnglish
Pages (from-to)682-690
Number of pages9
JournalBurns
Volume45
Issue number3
Early online date25 Oct 2018
DOIs
Publication statusPublished - May 2019

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Ephrin-A2
Ephrins
Ephrin-A5
Wound Healing
Cicatrix
Ligands
Wounds and Injuries
Collagen
Fibroblasts
Skin Neoplasms
Keratinocytes
Skin
Transgenic Mice
Hair Follicle
Dermis
Histology
Homeostasis

Cite this

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title = "Ephrin-A2 affects wound healing and scarring in a murine model of excisional injury",
abstract = "Ephrin ligand/Eph receptor signaling is important in both tissue development and homeostasis. There is increasing evidence that Ephrin/Eph signaling is important in the skin, involved in hair follicle cycling, epidermal differentiation, cutaneous innervation and skin cancer. However, there is currently limited information on the role of Ephrin/Eph signaling in cutaneous wound healing. Here we report the effects of the Ephrin-A2 and A5 ligands on wound healing. Using Ephrin-A2-/-, Ephrin-A5-/- and Ephrin-A2A5-/- transgenic mice, in vitro wound healing assays were conducted using isolated keratinocytes and fibroblasts. Ephrin-A2-/-, Ephrin-A2A5-/- and wild type mice with excisional wounds were used to analyze the impact of these ligands on wound closure, scar outcome, collagen orientation and re-innervation in vivo. The absence of the Ephrin-A2 and A5 ligands did not have any effect on dermal fibroblast proliferation or on fibroblast or keratinocyte migration. The loss of Ephrin-A2 and A5 ligands did not impact on the rate of wound closure or re-innervation after injury. However, changes in the gross morphology of the healed scar and in collagen histology of the scar dermis were observed in transgenic mice. Therefore Ephrin-A2 and A5 ligands may play an important role in final scar appearance associated with collagen deposition and structure.",
keywords = "Collagen, Ephrin A2, Keratinocyte, Scarring, Wound repair",
author = "Dulharie Wijeratne and Jennifer Rodger and Andrew Stevenson and Hilary Wallace and Pr{\^e}le, {Cecilia M} and Wood, {Fiona M} and Fear, {Mark W}",
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Ephrin-A2 affects wound healing and scarring in a murine model of excisional injury. / Wijeratne, Dulharie; Rodger, Jennifer; Stevenson, Andrew; Wallace, Hilary; Prêle, Cecilia M; Wood, Fiona M; Fear, Mark W.

In: Burns, Vol. 45, No. 3, 05.2019, p. 682-690.

Research output: Contribution to journalArticle

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T1 - Ephrin-A2 affects wound healing and scarring in a murine model of excisional injury

AU - Wijeratne, Dulharie

AU - Rodger, Jennifer

AU - Stevenson, Andrew

AU - Wallace, Hilary

AU - Prêle, Cecilia M

AU - Wood, Fiona M

AU - Fear, Mark W

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KW - Ephrin A2

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KW - Wound repair

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