TY - JOUR
T1 - EphA/ephrin-A interactions during optic nerve regeneration: restoration of topography and regulation of ephrin-A2 expression
AU - Rodger, Jennifer
AU - Vitale, P.N.
AU - Tee, Lisa
AU - King, C.E.
AU - Bartlett, Carole
AU - Fall, Abbie
AU - Brennan, C.
AU - O'Shea, James
AU - Dunlop, Sarah
AU - Beazley, Lyn
PY - 2004
Y1 - 2004
N2 - During visual system development, interactions between Eph tyrosine kinase receptors and their ligands, the ephrins, guide retinal ganglion cell (RGC) axons to their topographic targets in the optic tectum. Here we show that Eph/ephrin interactions are also involved in restoring topography during RGC axon regeneration in goldfish. Following optic nerve crush, EphA/ephrin-A interactions were blocked by intracranial injections of recombinant Epb receptor (EphA3-AP) or phosphoinositol phospholipase-C. Topographic errors with multiple inputs to some tectal loci were detected electrophysiologically and increased projections to caudal tectum demonstrated by RT-97 immunohistochemistry. In EpbA3-AP-injected fish, ephrin-A2-expressing cells in the retino-recipient tectal layers were reduced in number compared to controls and their distribution was no longer graded. The findings, supported by in vitro studies, implicate EphA/ephrin-A interactions in restoring precise topography and in regulating ephrin-A2 expression during regeneration. (C) 2003 Elsevier Inc. All rights reserved.
AB - During visual system development, interactions between Eph tyrosine kinase receptors and their ligands, the ephrins, guide retinal ganglion cell (RGC) axons to their topographic targets in the optic tectum. Here we show that Eph/ephrin interactions are also involved in restoring topography during RGC axon regeneration in goldfish. Following optic nerve crush, EphA/ephrin-A interactions were blocked by intracranial injections of recombinant Epb receptor (EphA3-AP) or phosphoinositol phospholipase-C. Topographic errors with multiple inputs to some tectal loci were detected electrophysiologically and increased projections to caudal tectum demonstrated by RT-97 immunohistochemistry. In EpbA3-AP-injected fish, ephrin-A2-expressing cells in the retino-recipient tectal layers were reduced in number compared to controls and their distribution was no longer graded. The findings, supported by in vitro studies, implicate EphA/ephrin-A interactions in restoring precise topography and in regulating ephrin-A2 expression during regeneration. (C) 2003 Elsevier Inc. All rights reserved.
U2 - 10.1016/j.mcn.2003.09.010
DO - 10.1016/j.mcn.2003.09.010
M3 - Article
C2 - 14962740
SN - 1044-7431
VL - 25
SP - 56
EP - 68
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 1
ER -