The present study further characterizes ascorbic acid-dependent I-125- ion binding in guinea-pig trachea. Binding of I-125- ion in the presence of ascorbic acid was detected at the epithelial/submucosal interface, apparently involving individual cells containing peroxidase enzyme. A similar binding pattern was observed when hydrogen peroxide was substituted for ascorbic acid. Binding could be inhibited by the addition of the H2O2 degrading enzyme catalase or the peroxidase inhibitor thiourea. Results demonstrated that this binding was dependent upon the addition of, or endogenous production of hydrogen peroxide and its metabolism via airway eosinophil peroxidase. The relevance of this anomalous iodine binding phenomenon to radioiodinated ligand binding studies is discussed.
|Journal||European Journal of Pharmacology (Environmental Toxicology and Pharmacology)|
|Publication status||Published - 1992|
Rigby, P., Self, G. J., & Goldie, R. (1992). EOSINOPHIL PEROXIDASE AND I-125(-) ION BINDING IN GUINEA-PIG TRACHEA. European Journal of Pharmacology (Environmental Toxicology and Pharmacology), 228, 141-146. https://doi.org/10.1016/0926-6917(92)90023-6