TY - JOUR
T1 - Enzyme-Linked Immunosorbent Assay for Campylobacter Pyloridis
T2 - Correlation with Presence of C. Pyloridis in the Gastric Mucosa
AU - Goodwin, C. Stewart
AU - Blincow, Elizabeth
AU - Peterson, Graeme
AU - Sanderson, Chris
AU - Cheng, Wendy
AU - Marshall, Barry
AU - Warren, J. Robin
AU - McCulloch, Ross
N1 - Funding Information:
Received for publication 28 April 1986, and in revised form 2 September 1986. This work was supported by a grant from the Royal Perth Hospital Research Foundation. Please address requests for reprints to Dr. C. S. Goodwin, Microbiology Department, Royal Perth Hospital, Box X 2213, GPO Perth, Western Australia, Australia.
PY - 1987/3
Y1 - 1987/3
N2 - Antibody to Campylobacter pyloridis was measured by ELISA in the sera of 160 patients from whom gastric biopsy specimens were also obtained. The antigen was an acid-glycine extract of C. pyloridis, and titers ranged from 80 to 22,000 ELISA units (EU). Of 117 patients in whom C. pyloridis was detected microhiologically or histologically, 87 (74%) had a titer ⩾300 EU, and only one had a titer `150 EU. Of 43 patients in whom C. pyloridis was not detected, only two (5%) had a titer b300 EU. Thus, for a titer of 300 EU the ELISA test had a specificity of 97% and a sensitivity of 81%. At 150 EU the specificity was 78%, and the sensitivity was 99%. Histological diagnosis of active chronic gastritis was associated with a high median ELISA titer (485 E), chronic gastritis with a much lower titer (150 EU), and normal histology with a titer of 110 EU. Discriminating use of this serological test could be of assistance to detect C. pyloridis in the gastric mucosa.
AB - Antibody to Campylobacter pyloridis was measured by ELISA in the sera of 160 patients from whom gastric biopsy specimens were also obtained. The antigen was an acid-glycine extract of C. pyloridis, and titers ranged from 80 to 22,000 ELISA units (EU). Of 117 patients in whom C. pyloridis was detected microhiologically or histologically, 87 (74%) had a titer ⩾300 EU, and only one had a titer `150 EU. Of 43 patients in whom C. pyloridis was not detected, only two (5%) had a titer b300 EU. Thus, for a titer of 300 EU the ELISA test had a specificity of 97% and a sensitivity of 81%. At 150 EU the specificity was 78%, and the sensitivity was 99%. Histological diagnosis of active chronic gastritis was associated with a high median ELISA titer (485 E), chronic gastritis with a much lower titer (150 EU), and normal histology with a titer of 110 EU. Discriminating use of this serological test could be of assistance to detect C. pyloridis in the gastric mucosa.
UR - http://www.scopus.com/inward/record.url?scp=0023152498&partnerID=8YFLogxK
U2 - 10.1093/infdis/155.3.488
DO - 10.1093/infdis/155.3.488
M3 - Article
C2 - 3805774
AN - SCOPUS:0023152498
SN - 0022-1899
VL - 155
SP - 488
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 3
ER -
