Enzymatic synthesis of peptides on a solid support

Rose Haddoub; Martin Dauner; Fiona A. Stefanowicz; Valeria Barattini; Nicolas Laurent; Sabine L. Flitsch

doi:10.1039/b816847d

Enzymatic synthesis of peptides on a solid support

Rose Haddoub, Martin Dauner, Fiona A. Stefanowicz, Valeria Barattini, Nicolas Laurent, Sabine L. Flitsch

Research output: Contribution to journal › Article › peer-review

15 Citations (Web of Science)

Abstract

We have previously shown that dipeptides can be synthesised in high yields from amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated amino acids as well as the synthesis of longer peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold.

Original language	English
Pages (from-to)	665-670
Number of pages	6
Journal	Organic & Biomolecular Chemistry
Volume	7
Issue number	4
DOIs	https://doi.org/10.1039/b816847d
Publication status	Published - 2009
Externally published	Yes

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10.1039/b816847d

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keywords = "SELF-ASSEMBLED MONOLAYERS, MASS-SPECTROMETRY, PROTEASE, GLYCOSYLATION",

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AU - Haddoub, Rose

AU - Dauner, Martin

AU - Stefanowicz, Fiona A.

AU - Barattini, Valeria

AU - Laurent, Nicolas

AU - Flitsch, Sabine L.

PY - 2009

Y1 - 2009

N2 - We have previously shown that dipeptides can be synthesised in high yields from amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated amino acids as well as the synthesis of longer peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold.

AB - We have previously shown that dipeptides can be synthesised in high yields from amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated amino acids as well as the synthesis of longer peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold.

KW - SELF-ASSEMBLED MONOLAYERS

KW - MASS-SPECTROMETRY

KW - PROTEASE

KW - GLYCOSYLATION

U2 - 10.1039/b816847d

DO - 10.1039/b816847d

M3 - Article

SN - 1477-0520

VL - 7

SP - 665

EP - 670

JO - Organic & Biomolecular Chemistry

JF - Organic & Biomolecular Chemistry

IS - 4

ER -

Enzymatic synthesis of peptides on a solid support

Abstract

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