ENU-induced mutagenesis reveals a role for the BH4 domain of Bcl-2 in bone homeostasis

Dian Teguh

    Research output: ThesisDoctoral Thesis

    100 Downloads (Pure)

    Abstract

    Osteoporosis is an aging-associated degenerative bone disease that occurs via the imbalanced activities of bone resorbing osteoclasts and bone forming osteoblasts, resulting in brittle bones susceptible to fractures. ENU-induced mutant mice were screened for bone phenotypes to identify novel molecules regulating bone homeostasis, leading to the discovery of a mouse line with an S24P mutation in the Bcl-2 gene BH4 domain, exhibiting aging and osteoporosis. The impact of this mutation was investigated ex vivo through analysis of the bone phenotype of the mice and in vitro through analysis of molecular and cellular activities of osteoclasts and osteoblasts from mutant mice.
    Original languageEnglish
    QualificationDoctorate
    Awarding Institution
    • The University of Western Australia
    Award date27 Jul 2017
    DOIs
    Publication statusUnpublished - 2017

      Fingerprint

    Cite this