Enhancing mosquito and arbovirus surveillance in Western Australia using genomics

Binit Lamichhane

doi:10.26182/sdbk-8n94

Enhancing mosquito and arbovirus surveillance in Western Australia using genomics

Research output: Thesis › Doctoral Thesis

Abstract

The arbovirus surveillance in Western Australia involves mosquito trapping, morphological classification, utilisation of RT-PCR, and, in some cases, virus culture to identify known alphaviruses and flaviviruses. This thesis aims to enhance arbovirus surveillance by employing advanced genomic technologies. The research involved barcoding 45 mosquito species, significantly adding WAmosquito barcodes to the mosquito barcoding initiative. Metatranscriptomics sequencing on six mosquito species unveiled a diverse virome, discovering 13 previously unknown viruses. In addition, the study genetically characterised 91 arboviruses from historical arbovirus surveillance samples. This thesis provides profound insights into the genetic diversity of mosquitoes and arboviruses circulating in the region.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Imrie, Allison, Supervisor Smith, David, Supervisor Shi, Mang, Supervisor, External person Michie, Alice, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	15 Aug 2024
DOIs	https://doi.org/10.26182/sdbk-8n94
Publication status	Unpublished - 2024

Embargo information

Embargoed from 15/08/2024 to 01/09/2025. Will become publicly avaliable on 01/09/2025

Access to Document

10.26182/sdbk-8n94

Embargoed Document

THESIS - DOCTOR OF PHILOSOPHY - LAMICHHANE Binit - 2024_
26.6 MB
Embargo ends: 1/09/25

1 Article

Metatranscriptomic Sequencing of Medically Important Mosquitoes Reveals Extensive Diversity of RNA Viruses and Other Microbial Communities in Western Australia
Lamichhane, B., Brockway, C., Evasco, K., Nicholson, J., Neville, P. J., Levy, A., Smith, D. & Imrie, A., Feb 2024, In: Pathogens. 13, 2, 107.
Research output: Contribution to journal › Article › peer-review

Open Access
2 Citations (Scopus)

Cite this

@phdthesis{6b0ac291db764f2ca4a89b3d83954d11,

title = "Enhancing mosquito and arbovirus surveillance in Western Australia using genomics",

abstract = "The arbovirus surveillance in Western Australia involves mosquito trapping, morphological classification, utilisation of RT-PCR, and, in some cases, virus culture to identify known alphaviruses and flaviviruses. This thesis aims to enhance arbovirus surveillance by employing advanced genomic technologies. The research involved barcoding 45 mosquito species, significantly adding WAmosquito barcodes to the mosquito barcoding initiative. Metatranscriptomics sequencing on six mosquito species unveiled a diverse virome, discovering 13 previously unknown viruses. In addition, the study genetically characterised 91 arboviruses from historical arbovirus surveillance samples. This thesis provides profound insights into the genetic diversity of mosquitoes and arboviruses circulating in the region.",

keywords = "arbovirus, mosquito, arboviruses, metatranscriptomics, DNA barcoding, insect-specific viruses",

author = "Binit Lamichhane",

year = "2024",

doi = "10.26182/sdbk-8n94",

language = "English",

school = "The University of Western Australia",

}

TY - BOOK

T1 - Enhancing mosquito and arbovirus surveillance in Western Australia using genomics

AU - Lamichhane, Binit

PY - 2024

Y1 - 2024

N2 - The arbovirus surveillance in Western Australia involves mosquito trapping, morphological classification, utilisation of RT-PCR, and, in some cases, virus culture to identify known alphaviruses and flaviviruses. This thesis aims to enhance arbovirus surveillance by employing advanced genomic technologies. The research involved barcoding 45 mosquito species, significantly adding WAmosquito barcodes to the mosquito barcoding initiative. Metatranscriptomics sequencing on six mosquito species unveiled a diverse virome, discovering 13 previously unknown viruses. In addition, the study genetically characterised 91 arboviruses from historical arbovirus surveillance samples. This thesis provides profound insights into the genetic diversity of mosquitoes and arboviruses circulating in the region.

AB - The arbovirus surveillance in Western Australia involves mosquito trapping, morphological classification, utilisation of RT-PCR, and, in some cases, virus culture to identify known alphaviruses and flaviviruses. This thesis aims to enhance arbovirus surveillance by employing advanced genomic technologies. The research involved barcoding 45 mosquito species, significantly adding WAmosquito barcodes to the mosquito barcoding initiative. Metatranscriptomics sequencing on six mosquito species unveiled a diverse virome, discovering 13 previously unknown viruses. In addition, the study genetically characterised 91 arboviruses from historical arbovirus surveillance samples. This thesis provides profound insights into the genetic diversity of mosquitoes and arboviruses circulating in the region.

KW - arbovirus

KW - mosquito

KW - arboviruses

KW - metatranscriptomics

KW - DNA barcoding

KW - insect-specific viruses

U2 - 10.26182/sdbk-8n94

DO - 10.26182/sdbk-8n94

M3 - Doctoral Thesis

ER -

Enhancing mosquito and arbovirus surveillance in Western Australia using genomics

Abstract

Embargo information

Access to Document

Embargoed Document

Fingerprint

Research output

Metatranscriptomic Sequencing of Medically Important Mosquitoes Reveals Extensive Diversity of RNA Viruses and Other Microbial Communities in Western Australia

Cite this