© Springer Science+Business Media New York 2014. Human induced pluripotent stem (iPS) cells are a promising source of autologous cardiomyocytes to repair and regenerate myocardium for treatment of heart disease. In this study, we describe a method for enhanced cardiomyocyte production from human iPS cells by treating embryoid bodies with a histone deacetylase inhibitor, trichostatin A (TSA), together with activin A and bone morphogenetic protein (BMP)-4. The resulting cardiomyocytes expressed cardiac-specific transcription factors and contractile proteins at both gene and protein levels. Functionally, the contractile embryoid bodies (EBs) displayed calcium cycling and were responsive to the chronotropic agents isoprenaline (0.1 μM) and carbachol (1 μM). The cardiomyocytes derived from human iPS cells may be used to engineer functional cardiac muscle tissue for studying pathophysiology of cardiac disease, for drug discovery test beds, and potentially for generation of cardiac grafts to surgically replace damaged myocardium.
|Title of host publication||Induced Pluripotent Stem (iPS) Cells: Methods and Protocols|
|Editors||Kursad Turksen, Andras Nagy|
|Number of pages||7|
|Publication status||Published - 1 Jan 2016|
|Name||Methods in Molecular Biology|
Lim, S. Y., Sivakumaran, P., Crombie, D. E., Dusting, G. J., Pébay, A., & Dilley, R. J. (2016). Enhancing human cardiomyocyte differentiation from induced pluripotent stem cells with trichostatin A. In K. Turksen, & A. Nagy (Eds.), Induced Pluripotent Stem (iPS) Cells: Methods and Protocols (Vol. 1357, pp. 415-421). (Methods in Molecular Biology). Humana Press. https://doi.org/10.1007/7651_2014_160