Natural pentatricopeptide repeat proteins are mostly insoluble and bind RNA in an unpredictable manner. We have designed a consensus PPR (cPPR) that is programmable, more stable and soluble. The cPPR binds to specific RNA depending on the choice of amino acids at position 4 and 34 of each cPPR repeat. I showed that cPPRs can be re-designed to block mitochondrial protein translation and microRNA. Interestingly cPPRs can also bind single-stranded DNA and regulate human telomerase in vitro. This is the first study on re-designing a natural RNA-binding protein to target ssDNA, mRNA and microRNA.
|Qualification||Doctor of Philosophy|
|Award date||7 Oct 2016|
|Publication status||Unpublished - 2016|
Take-down noticeEmbargoed from 11/10/16 to 16/11/18.
Made publicly available on 16/11/18.