Engineering pentatricopeptide repeat proteins for programmable nucleic acid manipulation

Tiong Sun Chia

    Research output: ThesisDoctoral Thesis

    74 Downloads (Pure)


    Natural pentatricopeptide repeat proteins are mostly insoluble and bind RNA in an unpredictable manner. We have designed a consensus PPR (cPPR) that is programmable, more stable and soluble. The cPPR binds to specific RNA depending on the choice of amino acids at position 4 and 34 of each cPPR repeat. I showed that cPPRs can be re-designed to block mitochondrial protein translation and microRNA. Interestingly cPPRs can also bind single-stranded DNA and regulate human telomerase in vitro. This is the first study on re-designing a natural RNA-binding protein to target ssDNA, mRNA and microRNA.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • The University of Western Australia
    • Rackham, Oliver, Supervisor
    • Filipovska, Aleksandra, Supervisor
    • Waterer, Grant, Supervisor
    Award date7 Oct 2016
    Publication statusUnpublished - 2016

    Embargo information

    • Embargoed from 11/10/16 to 16/11/18. Made publicly available on 16/11/18.


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