Engineering pentatricopeptide repeat proteins for programmable nucleic acid manipulation

Tiong Sun Chia

    Research output: ThesisDoctoral Thesis

    33 Downloads (Pure)

    Abstract

    Natural pentatricopeptide repeat proteins are mostly insoluble and bind RNA in an unpredictable manner. We have designed a consensus PPR (cPPR) that is programmable, more stable and soluble. The cPPR binds to specific RNA depending on the choice of amino acids at position 4 and 34 of each cPPR repeat. I showed that cPPRs can be re-designed to block mitochondrial protein translation and microRNA. Interestingly cPPRs can also bind single-stranded DNA and regulate human telomerase in vitro. This is the first study on re-designing a natural RNA-binding protein to target ssDNA, mRNA and microRNA.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • The University of Western Australia
    Award date7 Oct 2016
    Publication statusUnpublished - 2016

    Take-down notice

    Embargoed from 11/10/16 to 16/11/18.
    Made publicly available on 16/11/18.

    Fingerprint Dive into the research topics of 'Engineering pentatricopeptide repeat proteins for programmable nucleic acid manipulation'. Together they form a unique fingerprint.

  • Cite this