Engineering and characterisation of RNA-binding proteins using improved synthetic biology tools

As synthetic biology tools and devices become more refined and predictable. researchers are expanding the toolbox to important applications in RNA biology and RNA processing. In this work we bring together three important facets of synthetic biology as it relates to engineering RNA-binding proteins for post-transcriptional gene regulation, namely, a new platform for measuring the attenuated activity of RNA-binding proteins, insights into the structural and evolutionary principles that inform RNA-binding protein design, and a review of how these engineered proteins can help in dissecting post-transcriptional processes in mitochondria, a particularly challenging area of research.