TY - JOUR
T1 - Embryogenesis and plant regeneration of the perennial pasture and medicinal legume Bituminaria bituminosa (L.) C.H. Stirton
AU - Pazos Navarro, Maria
AU - Croser, Janine
AU - Castello, Marie-Claire
AU - Ramankutty, Padmaja
AU - Heel, Kathy
AU - Real Ferreiro, Daniel
AU - Correal, Enrique
AU - Dabauza, Mercedes
PY - 2014
Y1 - 2014
N2 - © 2014 CSIRO. Bituminaria bituminosa (common name tedera) is a drought-tolerant perennial pasture species of agronomic and pharmaceutical interest for Mediterranean climates. Considering the importance of this legume, in vitro experiments were conducted to develop protocols for plant regeneration from embryogenic calli of leaves, petioles and anthers to efficiently exploit and maintain selected important clones from the tedera breeding program. The type of explant was a key factor in the frequency of embryogenesis and the number of embryos per callus. For plant regeneration from cultured anthers, appropriate anther physiological state (uninucleate stage of microsporogenesis), stress treatments (electroporation, 25 Ω, 25F, 1500V) and culture conditions were determined. A robust flow-cytometry method was developed to analyse the ploidy status of callus, in vitro shoots and in vivo acclimatised plants derived from anther and leaf explants.
AB - © 2014 CSIRO. Bituminaria bituminosa (common name tedera) is a drought-tolerant perennial pasture species of agronomic and pharmaceutical interest for Mediterranean climates. Considering the importance of this legume, in vitro experiments were conducted to develop protocols for plant regeneration from embryogenic calli of leaves, petioles and anthers to efficiently exploit and maintain selected important clones from the tedera breeding program. The type of explant was a key factor in the frequency of embryogenesis and the number of embryos per callus. For plant regeneration from cultured anthers, appropriate anther physiological state (uninucleate stage of microsporogenesis), stress treatments (electroporation, 25 Ω, 25F, 1500V) and culture conditions were determined. A robust flow-cytometry method was developed to analyse the ploidy status of callus, in vitro shoots and in vivo acclimatised plants derived from anther and leaf explants.
U2 - 10.1071/CP14083
DO - 10.1071/CP14083
M3 - Article
SN - 1836-0947
VL - 65
SP - 934
EP - 943
JO - Crop and Pasture Science
JF - Crop and Pasture Science
IS - 9
ER -