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Abstract
The adsorption of serum proteins on the surface of nanoparticles (NPs) delivered into a biological environment has been known to alter NP surface properties and consequently their targeting efficiency. In this paper, we use random copolymer (p(HEMA-ran-GMA))-based NPs synthesized using 2-hydroxyethyl methacrylate (HEMA) and glycidyl methacrylate (GMA). We show that serum proteins bind to the NP and that functionalization with antibodies and peptides designed to facilitate NP passage across the blood-brain barrier (BBB) to bind specific cell types is ineffective. In particular, we use systematic in vitro and in vivo analyses to demonstrate that p(HEMA-ran-GMA) NPs functionalized with HIV-1 trans-activating transcriptor peptide (known to cross the BBB) and alpha neural/glial antigen 2 (NG2) (known for targeting oligodendrocyte precursor cells (OPCs)), individually and in combination, do not specifically target OPCs and are unable to cross the BBB, likely due to the serum protein binding to the NPs.
Original language | English |
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Pages (from-to) | 22085-22095 |
Number of pages | 11 |
Journal | ACS APPLIED MATERIALS & INTERFACES |
Volume | 11 |
Issue number | 25 |
DOIs | |
Publication status | Published - 26 Jun 2019 |
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Targeted nanoparticles to deliver combinations of calcium channel inhibitors to prevent myelin damage during secondary degeneration after neurotrauma
Fitzgerald, M. (Investigator 01), Dunlop, S. (Investigator 02), Swaminatha Iyer, I. (Investigator 03) & Smith, N. (Investigator 04)
NHMRC National Health and Medical Research Council
1/01/15 → 31/12/18
Project: Research
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Innovative and multi-disciplinary treatment strategies for secondary degeneration following neurotrauma
Fitzgerald, M. (Investigator 01)
NHMRC National Health and Medical Research Council
1/01/15 → 30/12/18
Project: Research