Elevated levels of circulating exosome in COPD patients are associated with systemic inflammation

Dino B.A. Tan, Jesse Armitage, Teck Hui Teo, Nathanael E. Ong, Heewoong Shin, Yuben P. Moodley

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Chronic obstructive pulmonary disease (COPD) is characterized by progressive pulmonary and systemic inflammation. Acute exacerbations of COPD (AECOPD) are associated with acute inflammation and infections and increase the rates of morbidity and mortality. Currently, neither the aetiology nor pathogenesis of AECOPD are entirely understood. Exosomes have been reported to regulate immunity and inflammation via specific intercellular communications through an array of macromolecules (e.g. microRNA and proteins) contained within these microvesicles. We evaluated the level of circulating exosomes in relation to systemic inflammation in patients with AECOPD (n = 20) or stable COPD (sCOPD; n = 20) in comparison to non-smoking healthy controls (n = 20). Exosomes in plasma were isolated by precipitation-based method, and quantified using a CD9 expression based enzyme-linked immunosorbent assay (ELISA). Plasma biomarkers of systemic inflammation, C-reactive protein (CRP), soluble tumour necrosis factor receptor-1 (sTNFR1) and interleukin (IL)-6 were also quantified using ELISA. Levels of plasma exosome were higher in AECOPD patients (p < 0.001) and sCOPD patients (p < 0.05) compared to controls. Plasma levels of CRP and sTNFR1 were highest in AECOPD, followed by sCOPD patients compared to healthy controls (p < 0.05). Plasma IL-6 was elevated in AECOPD (p < 0.05) and sCOPD patients (p < 0.01) compared to controls. The level of exosome correlated with the levels of CRP, sTNFR1 and IL-6 in plasma. Exosomes may therefore be involved in the inflammatory process of AECOPD. Further studies involving exosomal phenotyping and molecular characterization are required to fully understand their role in the pathophysiology of COPD.

Original languageEnglish
Pages (from-to)261-264
JournalRespiratory Medicine
Volume132
DOIs
Publication statusPublished - Nov 2017

Fingerprint

Exosomes
Chronic Obstructive Pulmonary Disease
Inflammation
Tumor Necrosis Factor Receptors
C-Reactive Protein
Interleukin-6
Enzyme-Linked Immunosorbent Assay
MicroRNAs
Immunity
Pneumonia
Biomarkers

Cite this

Tan, Dino B.A. ; Armitage, Jesse ; Teo, Teck Hui ; Ong, Nathanael E. ; Shin, Heewoong ; Moodley, Yuben P. / Elevated levels of circulating exosome in COPD patients are associated with systemic inflammation. In: Respiratory Medicine. 2017 ; Vol. 132. pp. 261-264.
@article{5ed18d0924134f1a8b60f6eaac05bebd,
title = "Elevated levels of circulating exosome in COPD patients are associated with systemic inflammation",
abstract = "Chronic obstructive pulmonary disease (COPD) is characterized by progressive pulmonary and systemic inflammation. Acute exacerbations of COPD (AECOPD) are associated with acute inflammation and infections and increase the rates of morbidity and mortality. Currently, neither the aetiology nor pathogenesis of AECOPD are entirely understood. Exosomes have been reported to regulate immunity and inflammation via specific intercellular communications through an array of macromolecules (e.g. microRNA and proteins) contained within these microvesicles. We evaluated the level of circulating exosomes in relation to systemic inflammation in patients with AECOPD (n = 20) or stable COPD (sCOPD; n = 20) in comparison to non-smoking healthy controls (n = 20). Exosomes in plasma were isolated by precipitation-based method, and quantified using a CD9 expression based enzyme-linked immunosorbent assay (ELISA). Plasma biomarkers of systemic inflammation, C-reactive protein (CRP), soluble tumour necrosis factor receptor-1 (sTNFR1) and interleukin (IL)-6 were also quantified using ELISA. Levels of plasma exosome were higher in AECOPD patients (p < 0.001) and sCOPD patients (p < 0.05) compared to controls. Plasma levels of CRP and sTNFR1 were highest in AECOPD, followed by sCOPD patients compared to healthy controls (p < 0.05). Plasma IL-6 was elevated in AECOPD (p < 0.05) and sCOPD patients (p < 0.01) compared to controls. The level of exosome correlated with the levels of CRP, sTNFR1 and IL-6 in plasma. Exosomes may therefore be involved in the inflammatory process of AECOPD. Further studies involving exosomal phenotyping and molecular characterization are required to fully understand their role in the pathophysiology of COPD.",
keywords = "AECOPD, COPD, Exosomes, Inflammation",
author = "Tan, {Dino B.A.} and Jesse Armitage and Teo, {Teck Hui} and Ong, {Nathanael E.} and Heewoong Shin and Moodley, {Yuben P.}",
year = "2017",
month = "11",
doi = "10.1016/j.rmed.2017.04.014",
language = "English",
volume = "132",
pages = "261--264",
journal = "Respiratory Medicine",
issn = "0954-6111",
publisher = "Saunders",

}

Elevated levels of circulating exosome in COPD patients are associated with systemic inflammation. / Tan, Dino B.A.; Armitage, Jesse; Teo, Teck Hui; Ong, Nathanael E.; Shin, Heewoong; Moodley, Yuben P.

In: Respiratory Medicine, Vol. 132, 11.2017, p. 261-264.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Elevated levels of circulating exosome in COPD patients are associated with systemic inflammation

AU - Tan, Dino B.A.

AU - Armitage, Jesse

AU - Teo, Teck Hui

AU - Ong, Nathanael E.

AU - Shin, Heewoong

AU - Moodley, Yuben P.

PY - 2017/11

Y1 - 2017/11

N2 - Chronic obstructive pulmonary disease (COPD) is characterized by progressive pulmonary and systemic inflammation. Acute exacerbations of COPD (AECOPD) are associated with acute inflammation and infections and increase the rates of morbidity and mortality. Currently, neither the aetiology nor pathogenesis of AECOPD are entirely understood. Exosomes have been reported to regulate immunity and inflammation via specific intercellular communications through an array of macromolecules (e.g. microRNA and proteins) contained within these microvesicles. We evaluated the level of circulating exosomes in relation to systemic inflammation in patients with AECOPD (n = 20) or stable COPD (sCOPD; n = 20) in comparison to non-smoking healthy controls (n = 20). Exosomes in plasma were isolated by precipitation-based method, and quantified using a CD9 expression based enzyme-linked immunosorbent assay (ELISA). Plasma biomarkers of systemic inflammation, C-reactive protein (CRP), soluble tumour necrosis factor receptor-1 (sTNFR1) and interleukin (IL)-6 were also quantified using ELISA. Levels of plasma exosome were higher in AECOPD patients (p < 0.001) and sCOPD patients (p < 0.05) compared to controls. Plasma levels of CRP and sTNFR1 were highest in AECOPD, followed by sCOPD patients compared to healthy controls (p < 0.05). Plasma IL-6 was elevated in AECOPD (p < 0.05) and sCOPD patients (p < 0.01) compared to controls. The level of exosome correlated with the levels of CRP, sTNFR1 and IL-6 in plasma. Exosomes may therefore be involved in the inflammatory process of AECOPD. Further studies involving exosomal phenotyping and molecular characterization are required to fully understand their role in the pathophysiology of COPD.

AB - Chronic obstructive pulmonary disease (COPD) is characterized by progressive pulmonary and systemic inflammation. Acute exacerbations of COPD (AECOPD) are associated with acute inflammation and infections and increase the rates of morbidity and mortality. Currently, neither the aetiology nor pathogenesis of AECOPD are entirely understood. Exosomes have been reported to regulate immunity and inflammation via specific intercellular communications through an array of macromolecules (e.g. microRNA and proteins) contained within these microvesicles. We evaluated the level of circulating exosomes in relation to systemic inflammation in patients with AECOPD (n = 20) or stable COPD (sCOPD; n = 20) in comparison to non-smoking healthy controls (n = 20). Exosomes in plasma were isolated by precipitation-based method, and quantified using a CD9 expression based enzyme-linked immunosorbent assay (ELISA). Plasma biomarkers of systemic inflammation, C-reactive protein (CRP), soluble tumour necrosis factor receptor-1 (sTNFR1) and interleukin (IL)-6 were also quantified using ELISA. Levels of plasma exosome were higher in AECOPD patients (p < 0.001) and sCOPD patients (p < 0.05) compared to controls. Plasma levels of CRP and sTNFR1 were highest in AECOPD, followed by sCOPD patients compared to healthy controls (p < 0.05). Plasma IL-6 was elevated in AECOPD (p < 0.05) and sCOPD patients (p < 0.01) compared to controls. The level of exosome correlated with the levels of CRP, sTNFR1 and IL-6 in plasma. Exosomes may therefore be involved in the inflammatory process of AECOPD. Further studies involving exosomal phenotyping and molecular characterization are required to fully understand their role in the pathophysiology of COPD.

KW - AECOPD

KW - COPD

KW - Exosomes

KW - Inflammation

UR - http://www.scopus.com/inward/record.url?scp=85018976259&partnerID=8YFLogxK

U2 - 10.1016/j.rmed.2017.04.014

DO - 10.1016/j.rmed.2017.04.014

M3 - Article

VL - 132

SP - 261

EP - 264

JO - Respiratory Medicine

JF - Respiratory Medicine

SN - 0954-6111

ER -