Elemental ultrastructure of bioleaching bacteria and archaea grown on different energy sources

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Abstract

The composition and distribution of elements within cells of two species of Fe and S oxidising microbes utilising S, Fe2+ or chalcopyrite (CuFeS2) as an energy source were compared to determine possible sites of oxidation and function of intracellular granules. The bacterium Acidithiobacillus ferrooxidans and the archaeon Metallosphaera hakonensis were examined using energy-filtered transmission electron microscopy (EFTEM), TEM energy dispersive X-ray spectroscopy (EDS), Scanning TEM (STEM) and electron energy-loss spectroscopy (EELS). Both species have intracellular granules and we show that these store Fe, S and P. The microbes slowly lost Fe from granules when switched to an Fe-free medium. EELS showed that the Fe in the granules of both species was consistent with Fe3+. Both microbes sometimes contain Cu and Si on their walls and intracellularly. Si concentrations have been shown to affect bioleach performance, so element deposition on the microbial catalyst may be a reason for this. Bands of Fe and S were present close to, or in, the cell membrane of M. hakonensis, as might be expected for the site of oxidation, and S also occurred throughout the cytoplasm. These are the first element maps of M. hakonensis, and these early results demonstrate that advanced characterisation and microanalysis techniques can provide insights into microbial processes involved in bioleaching.

Original languageEnglish
Title of host publicationBiohydrometallurgy 2009
Subtitle of host publicationA Meeting Point between Microbial Ecology, Metal Recovery Processes and Environmental Remediation - Selected, peer rev. papers 18th Int. Biohydrometallurgy Symp., IBS 2009
Pages235-238
Number of pages4
DOIs
Publication statusPublished - 28 Dec 2009
Event18th International Biohydrometallurgy Symposium, IBS 2009 - Bariloche, Argentina
Duration: 13 Sep 200917 Sep 2009

Publication series

NameAdvanced Materials Research
Volume71-73
ISSN (Print)1022-6680

Conference

Conference18th International Biohydrometallurgy Symposium, IBS 2009
Country/TerritoryArgentina
CityBariloche
Period13/09/0917/09/09

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