Elemental and isotopic imaging of biological samples using NanoSIMS

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With its low detection limits and the ability to analyze most of the elements in the periodic table, secondary ion mass spectrometry (SIMS) represents one of the most versatile in situ analytical techniques available, and recent developments have resulted in significant advantages for the use of imaging mass spectrometry in biological and biomedical research. Increases in spatial resolution and sensitivity allow detailed interrogation of samples at relevant scales and chemical concentrations. Advances in dynamic SIMS, specifically with the advent of NanoSIMS, now allow the tracking of stable isotopes within biological systems at subcellular length scales, while static SIMS combines subcellular imaging with molecular identification. In this chapter, we present an introduction to the SIMS technique, with particular reference to NanoSIMS, and discuss its application in biological and biomedical research. © Springer Science+Business Media New York 2014.
Original languageEnglish
Title of host publicationElectron Microscopy: Methods and Protocols
EditorsJohn Kuo
Place of PublicationNew York
ISBN (Print)9781627037761
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology


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