Electrochemiluminescence methods using CdS quantum dots in aptamer-based thrombin biosensors: a comparative study

Ibrahim Isildak, Farzaneh Navaeipour, Hadi Afsharan, Gulsah Soydan Kanberoglu, Ismail Agir, Tugba Ozer, Nasim Annabi, Eugenia Eftimie Totu, Balal Khalilzadeh

Research output: Contribution to journalArticlepeer-review

39 Citations (Web of Science)

Abstract

The detection of thrombin by using CdS nanocrystals (CdS NCs), gold nanoparticles (AuNPs) and luminol is investigated in this work. Thrombin is detected by three methods. One is called the quenching method. It is based on the quenching effect of AuNPs on the yellow fluorescence of CdS NCs (with excitation/emission wavelengths of 355/550 nm) when placed adjacent to CdS NCs. The second method (called amplification method) is based on an amplification mechanism in which the plasmonics on the AuNPs enhance the emission of CdS NCs through distance related Förster resonance energy transfer (FRET). The third method is ratiometric and based on the emission by two luminophores, viz. CdS NCs and luminol. In this method, by increasing the concentration of thrombin, the intensity of CdS NCs decreases, while that of luminol increases. The results showed that ratiometric method was most sensitive (with an LOD of 500 fg.mL-1), followed by the amplification method (6.5 pg.mL-1) and the quenching method (92 pg.mL-1). Hence, the latter is less useful. [Figure not available: see fulltext.].

Original languageEnglish
Article number25
JournalMicrochimica Acta
Volume187
Issue number1
DOIs
Publication statusPublished - 1 Jan 2020
Externally publishedYes

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