Effects of pH and bicarbonate on the nutrient status and growth of three Lupinus species

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Abstract

Aims: High pH, and high bicarbonate (HCO 3 ) and calcium (Ca) availability characterise calcareous soils. High [Ca] only partially explains why some Lupinus species are calcifuge, so we explored high [HCO 3 ] and high pH. Methods: We grew six Lupinus genotypes in hydroponics with pH 5, 6.5 and 8 a (adjusted by KOH), and 8 b (adjusted by KHCO 3 ). Leaf symptoms and areas, root appearance and biomass were recorded; whole leaf and root nutrient concentrations, and leaf cellular phosphorus (P), Ca and potassium (K) concentrations were determined using elemental X-ray microanalysis. Results: Chlorosis was observed in young leaves at high pH for L. angustifolius and L. cosentinii, and P deficiency at high pH for all genotypes. High pH decreased iron (Fe) and zinc (Zn) uptake in all genotypes. It also decreased lateral root growth, the uptake of P, K, Ca, and manganese (Mn) by all sensitive species; and translocation of P, Fe, Zn, Mn, and Ca to leaves in most sensitive species. However, leaf [Ca], leaf [K], [K] within each measured cell type, and translocation of K and Ca to leaves of L. pilosus and L. cosentinii at pH 8 were greater than at pH 5 and 6.5. Compared with pH 8 a , all L. angustifolius genotypes translocated more P, Fe, Zn, Mn and K from roots to leaves at pH 8 b . High pH did not affect the leaf cell types that accumulated P and Ca, but decreased the leaf cellular [P]. Conclusions: Lupinus angustifolius and L. cosentinii were sensitive to high [HCO 3 ] and/or high pH; L. pilosus was relatively tolerant. High pH decreased lateral root growth and nutrient uptake, inhibiting growth of sensitive species. High [HCO 3 ] diminished the negative effect of pH 8 on nutrient translocation to leaves in most L. angustifolius genotypes. This knowledge provides critical insights into the habits of Lupinus species to guide breeding of calcicole plants.

Original languageEnglish
Number of pages20
JournalPlant and Soil
DOIs
Publication statusE-pub ahead of print - 14 Feb 2019

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Lupinus
bicarbonates
bicarbonate
nutrient
nutrients
calcium
leaves
genotype
translocation
manganese
Lupinus angustifolius
zinc
effect
root growth
calcicoles
uptake mechanisms
hydroponics
calcareous soil
calcareous soils
nutrient uptake

Cite this

@article{512d153ce41b430f898aad3a26d46748,
title = "Effects of pH and bicarbonate on the nutrient status and growth of three Lupinus species",
abstract = "Aims: High pH, and high bicarbonate (HCO 3 − ) and calcium (Ca) availability characterise calcareous soils. High [Ca] only partially explains why some Lupinus species are calcifuge, so we explored high [HCO 3 − ] and high pH. Methods: We grew six Lupinus genotypes in hydroponics with pH 5, 6.5 and 8 a (adjusted by KOH), and 8 b (adjusted by KHCO 3 ). Leaf symptoms and areas, root appearance and biomass were recorded; whole leaf and root nutrient concentrations, and leaf cellular phosphorus (P), Ca and potassium (K) concentrations were determined using elemental X-ray microanalysis. Results: Chlorosis was observed in young leaves at high pH for L. angustifolius and L. cosentinii, and P deficiency at high pH for all genotypes. High pH decreased iron (Fe) and zinc (Zn) uptake in all genotypes. It also decreased lateral root growth, the uptake of P, K, Ca, and manganese (Mn) by all sensitive species; and translocation of P, Fe, Zn, Mn, and Ca to leaves in most sensitive species. However, leaf [Ca], leaf [K], [K] within each measured cell type, and translocation of K and Ca to leaves of L. pilosus and L. cosentinii at pH 8 were greater than at pH 5 and 6.5. Compared with pH 8 a , all L. angustifolius genotypes translocated more P, Fe, Zn, Mn and K from roots to leaves at pH 8 b . High pH did not affect the leaf cell types that accumulated P and Ca, but decreased the leaf cellular [P]. Conclusions: Lupinus angustifolius and L. cosentinii were sensitive to high [HCO 3 − ] and/or high pH; L. pilosus was relatively tolerant. High pH decreased lateral root growth and nutrient uptake, inhibiting growth of sensitive species. High [HCO 3 − ] diminished the negative effect of pH 8 on nutrient translocation to leaves in most L. angustifolius genotypes. This knowledge provides critical insights into the habits of Lupinus species to guide breeding of calcicole plants.",
keywords = "High bicarbonate, High pH, Lateral root growth, Leaf chlorosis, Phosphorus deficiency, X-ray microanalysis",
author = "Wenli Ding and Clode, {Peta L.} and Hans Lambers",
year = "2019",
month = "2",
day = "14",
doi = "10.1007/s11104-019-03980-8",
language = "English",
journal = "Plant and Soil: An International Journal on Plant-Soil Relationships",
issn = "0032-079X",
publisher = "Springer",

}

TY - JOUR

T1 - Effects of pH and bicarbonate on the nutrient status and growth of three Lupinus species

AU - Ding, Wenli

AU - Clode, Peta L.

AU - Lambers, Hans

PY - 2019/2/14

Y1 - 2019/2/14

N2 - Aims: High pH, and high bicarbonate (HCO 3 − ) and calcium (Ca) availability characterise calcareous soils. High [Ca] only partially explains why some Lupinus species are calcifuge, so we explored high [HCO 3 − ] and high pH. Methods: We grew six Lupinus genotypes in hydroponics with pH 5, 6.5 and 8 a (adjusted by KOH), and 8 b (adjusted by KHCO 3 ). Leaf symptoms and areas, root appearance and biomass were recorded; whole leaf and root nutrient concentrations, and leaf cellular phosphorus (P), Ca and potassium (K) concentrations were determined using elemental X-ray microanalysis. Results: Chlorosis was observed in young leaves at high pH for L. angustifolius and L. cosentinii, and P deficiency at high pH for all genotypes. High pH decreased iron (Fe) and zinc (Zn) uptake in all genotypes. It also decreased lateral root growth, the uptake of P, K, Ca, and manganese (Mn) by all sensitive species; and translocation of P, Fe, Zn, Mn, and Ca to leaves in most sensitive species. However, leaf [Ca], leaf [K], [K] within each measured cell type, and translocation of K and Ca to leaves of L. pilosus and L. cosentinii at pH 8 were greater than at pH 5 and 6.5. Compared with pH 8 a , all L. angustifolius genotypes translocated more P, Fe, Zn, Mn and K from roots to leaves at pH 8 b . High pH did not affect the leaf cell types that accumulated P and Ca, but decreased the leaf cellular [P]. Conclusions: Lupinus angustifolius and L. cosentinii were sensitive to high [HCO 3 − ] and/or high pH; L. pilosus was relatively tolerant. High pH decreased lateral root growth and nutrient uptake, inhibiting growth of sensitive species. High [HCO 3 − ] diminished the negative effect of pH 8 on nutrient translocation to leaves in most L. angustifolius genotypes. This knowledge provides critical insights into the habits of Lupinus species to guide breeding of calcicole plants.

AB - Aims: High pH, and high bicarbonate (HCO 3 − ) and calcium (Ca) availability characterise calcareous soils. High [Ca] only partially explains why some Lupinus species are calcifuge, so we explored high [HCO 3 − ] and high pH. Methods: We grew six Lupinus genotypes in hydroponics with pH 5, 6.5 and 8 a (adjusted by KOH), and 8 b (adjusted by KHCO 3 ). Leaf symptoms and areas, root appearance and biomass were recorded; whole leaf and root nutrient concentrations, and leaf cellular phosphorus (P), Ca and potassium (K) concentrations were determined using elemental X-ray microanalysis. Results: Chlorosis was observed in young leaves at high pH for L. angustifolius and L. cosentinii, and P deficiency at high pH for all genotypes. High pH decreased iron (Fe) and zinc (Zn) uptake in all genotypes. It also decreased lateral root growth, the uptake of P, K, Ca, and manganese (Mn) by all sensitive species; and translocation of P, Fe, Zn, Mn, and Ca to leaves in most sensitive species. However, leaf [Ca], leaf [K], [K] within each measured cell type, and translocation of K and Ca to leaves of L. pilosus and L. cosentinii at pH 8 were greater than at pH 5 and 6.5. Compared with pH 8 a , all L. angustifolius genotypes translocated more P, Fe, Zn, Mn and K from roots to leaves at pH 8 b . High pH did not affect the leaf cell types that accumulated P and Ca, but decreased the leaf cellular [P]. Conclusions: Lupinus angustifolius and L. cosentinii were sensitive to high [HCO 3 − ] and/or high pH; L. pilosus was relatively tolerant. High pH decreased lateral root growth and nutrient uptake, inhibiting growth of sensitive species. High [HCO 3 − ] diminished the negative effect of pH 8 on nutrient translocation to leaves in most L. angustifolius genotypes. This knowledge provides critical insights into the habits of Lupinus species to guide breeding of calcicole plants.

KW - High bicarbonate

KW - High pH

KW - Lateral root growth

KW - Leaf chlorosis

KW - Phosphorus deficiency

KW - X-ray microanalysis

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U2 - 10.1007/s11104-019-03980-8

DO - 10.1007/s11104-019-03980-8

M3 - Article

JO - Plant and Soil: An International Journal on Plant-Soil Relationships

JF - Plant and Soil: An International Journal on Plant-Soil Relationships

SN - 0032-079X

ER -