Effects of intra-amniotic lipopolysaccharide exposure on the fetal lamb lung as gestation advances

V.A.C. Lambermont, E. Kuypers, J.J.P. Collins, Jane Pillow, John Newnham, Graeme Polglase, Ilias Nitsos, Matthew Kemp, Alan Jobe, Suhas Kallapur, Boris Kramer

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Intra-amniotic lipopolysaccharide (LPS) exposure may affect neonatal outcome by altering fetal lung and immune system development. We hypothesized that intra-amniotic LPS exposure would cause persistent fetal pulmonary responses as the lungs develop in utero. Methods: Fetal lambs were exposed to intra-amniotic LPS at 118 or at 118 and 123 d of gestational age (GA) with delivery at 125, 133, or 140 d (term = 147 d). Immune responses, PU.1 expression, Toll-like receptor (TLR)-1,2,4,6 mRNA levels, mast cell levels, and pulmonary elastin deposition were evaluated. Results: After a single dose of LPS, pulmonary inflammatory responses were observed with increases of (i) PU.1 and TLR1 at 125 d GA and (ii) monocytes, lymphocytes, TLR2, and TLR6 at 133 d GA. Repetitive LPS exposure resulted in (i) increases of neutrophils, monocytes, PU.1, and TLR1 at 125 d GA; (ii) increases of neutrophils, PU.1, and TLR2 at 133 d GA; and (iii) decreases of mast cells, elastin foci, TLR4, and TLR6 at early gestation. At 140 d GA, only PU.1 was increased after repetitive LPS exposure. Conclusion: The preterm fetal lung can respond to a single exposure or repeated exposures from intra-amniotic LPS in multiple ways, but the absence of inflammatory and structural changes in LPS-exposed fetuses delivered near term suggest that the fetus can resolve an inflammatory stimulus in utero with time. Copyright © 2014 International Pediatric Research Foundation, Inc.
Original languageEnglish
Pages (from-to)500-506
JournalPediatric Research
Volume75
Issue number4
DOIs
Publication statusPublished - 2014

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