Effects of epidermal growth factor and mitogenic pathway inhibitors on rat colon cancer proliferation in vitro

D. Lopez, Phillip Oates, Anthony House

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The proliferative responses of two rat colon cancer cell lines (Per192NR and Per237) to concentrations of epidermal growth factor (EGF) were assessed alone, or in combination with calcium sequestration or inhibitors of protein kinase A, C (PKA and PKC). Up to 160nM of EGF stimulated cell proliferation in Per237 cells, but was ineffective in Per192NR cells. In both cell lines all inhibitors failed to alter basal proliferation. In Per192NR cells the combination of 5 to 20nM EGF and inhibitor resulted in a biphasic reduction in basal proliferation which was lost by 20nM. In the Per237 cells only 10nM EGF and PKA inhibitor reduced proliferation. Tumours derived from the same origin respond differently to EGF-induced mitogenesis.
Original languageEnglish
Pages (from-to)157-162
JournalAnticancer Research
Volume15
Publication statusPublished - 1995

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Epidermal Growth Factor
Colonic Neoplasms
Cell Line
Cyclic AMP-Dependent Protein Kinases
Protein Kinase C
Cell Proliferation
In Vitro Techniques
Calcium
Neoplasms

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title = "Effects of epidermal growth factor and mitogenic pathway inhibitors on rat colon cancer proliferation in vitro",
abstract = "The proliferative responses of two rat colon cancer cell lines (Per192NR and Per237) to concentrations of epidermal growth factor (EGF) were assessed alone, or in combination with calcium sequestration or inhibitors of protein kinase A, C (PKA and PKC). Up to 160nM of EGF stimulated cell proliferation in Per237 cells, but was ineffective in Per192NR cells. In both cell lines all inhibitors failed to alter basal proliferation. In Per192NR cells the combination of 5 to 20nM EGF and inhibitor resulted in a biphasic reduction in basal proliferation which was lost by 20nM. In the Per237 cells only 10nM EGF and PKA inhibitor reduced proliferation. Tumours derived from the same origin respond differently to EGF-induced mitogenesis.",
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journal = "Anticancer Research",
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Effects of epidermal growth factor and mitogenic pathway inhibitors on rat colon cancer proliferation in vitro. / Lopez, D.; Oates, Phillip; House, Anthony.

In: Anticancer Research, Vol. 15, 1995, p. 157-162.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of epidermal growth factor and mitogenic pathway inhibitors on rat colon cancer proliferation in vitro

AU - Lopez, D.

AU - Oates, Phillip

AU - House, Anthony

PY - 1995

Y1 - 1995

N2 - The proliferative responses of two rat colon cancer cell lines (Per192NR and Per237) to concentrations of epidermal growth factor (EGF) were assessed alone, or in combination with calcium sequestration or inhibitors of protein kinase A, C (PKA and PKC). Up to 160nM of EGF stimulated cell proliferation in Per237 cells, but was ineffective in Per192NR cells. In both cell lines all inhibitors failed to alter basal proliferation. In Per192NR cells the combination of 5 to 20nM EGF and inhibitor resulted in a biphasic reduction in basal proliferation which was lost by 20nM. In the Per237 cells only 10nM EGF and PKA inhibitor reduced proliferation. Tumours derived from the same origin respond differently to EGF-induced mitogenesis.

AB - The proliferative responses of two rat colon cancer cell lines (Per192NR and Per237) to concentrations of epidermal growth factor (EGF) were assessed alone, or in combination with calcium sequestration or inhibitors of protein kinase A, C (PKA and PKC). Up to 160nM of EGF stimulated cell proliferation in Per237 cells, but was ineffective in Per192NR cells. In both cell lines all inhibitors failed to alter basal proliferation. In Per192NR cells the combination of 5 to 20nM EGF and inhibitor resulted in a biphasic reduction in basal proliferation which was lost by 20nM. In the Per237 cells only 10nM EGF and PKA inhibitor reduced proliferation. Tumours derived from the same origin respond differently to EGF-induced mitogenesis.

M3 - Article

VL - 15

SP - 157

EP - 162

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

ER -