Effects of dietary leucine and phenylalanine on gastrointestinal development and small intestinal enzyme activities in milk-fed holstein dairy calves

Yangchun Cao, Shimin Liu, Xinjian Yang, Long Guo, Chuanjiang Cai, Junhu Yao

Research output: Contribution to journalArticle

Abstract

This study was investigated the effects of dietary supplementation of leucine and phenylalanine on the development of the gastrointestinal tract and the intestinal digestive enzyme activity in male Holstein dairy calves. Twenty calves with a body weight of 38 + 3 kg at 1 day of age were randomly divided into four groups: a control group, a leucine group (1.435 g · l −1 ), a phenylalanine group (0.725 g · l −1 ), and a mixed amino acid group (1.435 g · l −1 leucine plus 0.725 g · l −1 phenylalanine). The supplementation of leucine decreased the short-circuit current (Isc) of the rumen and duodenum (P<0.01); phenylalanine did not show any influence on the Isc of rumen and duodenum (P>0.05), and also counteracted the Isc reduction caused by leucine. Leucine increased the trypsin activity at the 20% relative site of the small intestine (P<0.05). There was no difference in the activity of α-amylase and of lactase in the small intestinal chyme among four treatments (P>0.05). The trypsin activity in the anterior segment of the small intestine was higher than other segments, whereas the α-amylase activity in the posterior segment of the small intestine was higher than other segments. Leucine can reduce Isc of the rumen and duodenum, improve the development of the gastrointestinal tract, and enhance trypsin activity; phenylalanine could inhibit the effect of leucine in promoting intestinal development.

Original languageEnglish
Article numberBSR20181733
JournalBioscience Reports
Volume39
Issue number1
DOIs
Publication statusPublished - 25 Jan 2019

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Dairies
Enzyme activity
Phenylalanine
Leucine
Milk
Enzymes
Trypsin
Small Intestine
Rumen
Duodenum
Gastrointestinal Tract
Amylases
Dietary Supplements
Short circuit currents
Body Weight
Amino Acids
Control Groups

Cite this

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title = "Effects of dietary leucine and phenylalanine on gastrointestinal development and small intestinal enzyme activities in milk-fed holstein dairy calves",
abstract = "This study was investigated the effects of dietary supplementation of leucine and phenylalanine on the development of the gastrointestinal tract and the intestinal digestive enzyme activity in male Holstein dairy calves. Twenty calves with a body weight of 38 + − 3 kg at 1 day of age were randomly divided into four groups: a control group, a leucine group (1.435 g · l −1 ), a phenylalanine group (0.725 g · l −1 ), and a mixed amino acid group (1.435 g · l −1 leucine plus 0.725 g · l −1 phenylalanine). The supplementation of leucine decreased the short-circuit current (Isc) of the rumen and duodenum (P<0.01); phenylalanine did not show any influence on the Isc of rumen and duodenum (P>0.05), and also counteracted the Isc reduction caused by leucine. Leucine increased the trypsin activity at the 20{\%} relative site of the small intestine (P<0.05). There was no difference in the activity of α-amylase and of lactase in the small intestinal chyme among four treatments (P>0.05). The trypsin activity in the anterior segment of the small intestine was higher than other segments, whereas the α-amylase activity in the posterior segment of the small intestine was higher than other segments. Leucine can reduce Isc of the rumen and duodenum, improve the development of the gastrointestinal tract, and enhance trypsin activity; phenylalanine could inhibit the effect of leucine in promoting intestinal development.",
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Effects of dietary leucine and phenylalanine on gastrointestinal development and small intestinal enzyme activities in milk-fed holstein dairy calves. / Cao, Yangchun; Liu, Shimin; Yang, Xinjian; Guo, Long; Cai, Chuanjiang; Yao, Junhu.

In: Bioscience Reports, Vol. 39, No. 1, BSR20181733, 25.01.2019.

Research output: Contribution to journalArticle

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T1 - Effects of dietary leucine and phenylalanine on gastrointestinal development and small intestinal enzyme activities in milk-fed holstein dairy calves

AU - Cao, Yangchun

AU - Liu, Shimin

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AU - Guo, Long

AU - Cai, Chuanjiang

AU - Yao, Junhu

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