Effects of dietary acute tryptophan depletion (ATD) on NPY serum levels in healthy adult humans whilst controlling for methionine supply—a pilot study

Janice W.Y. Wong, Hugo A.E. Morandini, Vita L.S. Dingerkus, Tilman J. Gaber, Kevin C. Runions, Pradeep Rao, Simone Mahfouda, Katrin Helmbold, Sarah Bubenzer-Busch, Rebecca Koenemann, Richard M. Stewart, Florian D. Zepf

Research output: Contribution to journalArticle

Abstract

Central nervous serotonin (5-HT) can influence behaviour and neuropsychiatric disorders. Evidence from animal models suggest that lowered levels of neuropeptide Y (NPY) may have similar effects, although it is currently unknown whether decreased central nervous 5-HT impact NPY concentrations. Given that the production of NPY is dependent on the essential amino acid methionine (MET), it is imperative to account for the presence of MET in such investigations. Hence, this study sought to examine the effects of acute tryptophan depletion (ATD; a dietary procedure that temporarily lowers central nervous 5-HT synthesis) on serum concentrations of NPY, whilst using the potential renal acid load indicator (PRAL) to control for levels of MET. In a double-blind repeated measures design, 24 adult humans randomly received an AA-load lacking in TRP (ATD) on one occasion, and a balanced control mixture with TRP (BAL) on a second occasion, both with a PRAL of nearly 47.3 mEq of MET. Blood samples were obtained at 90, 180, and 240 min after each of the AA challenges. ATD, and therefore, diminished substrate availability for brain 5-HT synthesis did not lead to significant changes in serum NPY concentrations over time, compared to BAL, under an acute acidotic stimulus.

Original languageEnglish
Article number594
Number of pages12
JournalNutrients
Volume10
Issue number5
DOIs
Publication statusPublished - 11 May 2018

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neuropeptide Y
Neuropeptide Y
blood serum
serotonin
Tryptophan
Methionine
tryptophan
methionine
Serotonin
Dimercaprol
Serum
kidneys
Kidney
Acids
synthesis
Essential Amino Acids
acids
essential amino acids
Animal Models
animal models

Cite this

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title = "Effects of dietary acute tryptophan depletion (ATD) on NPY serum levels in healthy adult humans whilst controlling for methionine supply—a pilot study",
abstract = "Central nervous serotonin (5-HT) can influence behaviour and neuropsychiatric disorders. Evidence from animal models suggest that lowered levels of neuropeptide Y (NPY) may have similar effects, although it is currently unknown whether decreased central nervous 5-HT impact NPY concentrations. Given that the production of NPY is dependent on the essential amino acid methionine (MET), it is imperative to account for the presence of MET in such investigations. Hence, this study sought to examine the effects of acute tryptophan depletion (ATD; a dietary procedure that temporarily lowers central nervous 5-HT synthesis) on serum concentrations of NPY, whilst using the potential renal acid load indicator (PRAL) to control for levels of MET. In a double-blind repeated measures design, 24 adult humans randomly received an AA-load lacking in TRP (ATD) on one occasion, and a balanced control mixture with TRP (BAL) on a second occasion, both with a PRAL of nearly 47.3 mEq of MET. Blood samples were obtained at 90, 180, and 240 min after each of the AA challenges. ATD, and therefore, diminished substrate availability for brain 5-HT synthesis did not lead to significant changes in serum NPY concentrations over time, compared to BAL, under an acute acidotic stimulus.",
keywords = "Acid base status, Mental health, Methionine, Neuropeptide Y, Serotonin depletion, Tryptophan",
author = "Wong, {Janice W.Y.} and Morandini, {Hugo A.E.} and Dingerkus, {Vita L.S.} and Gaber, {Tilman J.} and Runions, {Kevin C.} and Pradeep Rao and Simone Mahfouda and Katrin Helmbold and Sarah Bubenzer-Busch and Rebecca Koenemann and Stewart, {Richard M.} and Zepf, {Florian D.}",
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Effects of dietary acute tryptophan depletion (ATD) on NPY serum levels in healthy adult humans whilst controlling for methionine supply—a pilot study. / Wong, Janice W.Y.; Morandini, Hugo A.E.; Dingerkus, Vita L.S.; Gaber, Tilman J.; Runions, Kevin C.; Rao, Pradeep; Mahfouda, Simone; Helmbold, Katrin; Bubenzer-Busch, Sarah; Koenemann, Rebecca; Stewart, Richard M.; Zepf, Florian D.

In: Nutrients, Vol. 10, No. 5, 594, 11.05.2018.

Research output: Contribution to journalArticle

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T1 - Effects of dietary acute tryptophan depletion (ATD) on NPY serum levels in healthy adult humans whilst controlling for methionine supply—a pilot study

AU - Wong, Janice W.Y.

AU - Morandini, Hugo A.E.

AU - Dingerkus, Vita L.S.

AU - Gaber, Tilman J.

AU - Runions, Kevin C.

AU - Rao, Pradeep

AU - Mahfouda, Simone

AU - Helmbold, Katrin

AU - Bubenzer-Busch, Sarah

AU - Koenemann, Rebecca

AU - Stewart, Richard M.

AU - Zepf, Florian D.

PY - 2018/5/11

Y1 - 2018/5/11

N2 - Central nervous serotonin (5-HT) can influence behaviour and neuropsychiatric disorders. Evidence from animal models suggest that lowered levels of neuropeptide Y (NPY) may have similar effects, although it is currently unknown whether decreased central nervous 5-HT impact NPY concentrations. Given that the production of NPY is dependent on the essential amino acid methionine (MET), it is imperative to account for the presence of MET in such investigations. Hence, this study sought to examine the effects of acute tryptophan depletion (ATD; a dietary procedure that temporarily lowers central nervous 5-HT synthesis) on serum concentrations of NPY, whilst using the potential renal acid load indicator (PRAL) to control for levels of MET. In a double-blind repeated measures design, 24 adult humans randomly received an AA-load lacking in TRP (ATD) on one occasion, and a balanced control mixture with TRP (BAL) on a second occasion, both with a PRAL of nearly 47.3 mEq of MET. Blood samples were obtained at 90, 180, and 240 min after each of the AA challenges. ATD, and therefore, diminished substrate availability for brain 5-HT synthesis did not lead to significant changes in serum NPY concentrations over time, compared to BAL, under an acute acidotic stimulus.

AB - Central nervous serotonin (5-HT) can influence behaviour and neuropsychiatric disorders. Evidence from animal models suggest that lowered levels of neuropeptide Y (NPY) may have similar effects, although it is currently unknown whether decreased central nervous 5-HT impact NPY concentrations. Given that the production of NPY is dependent on the essential amino acid methionine (MET), it is imperative to account for the presence of MET in such investigations. Hence, this study sought to examine the effects of acute tryptophan depletion (ATD; a dietary procedure that temporarily lowers central nervous 5-HT synthesis) on serum concentrations of NPY, whilst using the potential renal acid load indicator (PRAL) to control for levels of MET. In a double-blind repeated measures design, 24 adult humans randomly received an AA-load lacking in TRP (ATD) on one occasion, and a balanced control mixture with TRP (BAL) on a second occasion, both with a PRAL of nearly 47.3 mEq of MET. Blood samples were obtained at 90, 180, and 240 min after each of the AA challenges. ATD, and therefore, diminished substrate availability for brain 5-HT synthesis did not lead to significant changes in serum NPY concentrations over time, compared to BAL, under an acute acidotic stimulus.

KW - Acid base status

KW - Mental health

KW - Methionine

KW - Neuropeptide Y

KW - Serotonin depletion

KW - Tryptophan

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DO - 10.3390/nu10050594

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JO - Nutrients

JF - Nutrients

SN - 2072-6643

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ER -