Effects of a myosin-II inhibitor (N-benzyl-p-toluene sulphonamide, BTS) on contractile characteristics of intact fast-twitch mammalian muscle fibres

Gavin Pinniger, J.D. Bruton, H. Westerblad, K.W. Ranatunga

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    28 Citations (Scopus)

    Abstract

    We have examined the effects of N-benzyl-p-toluene sulphonamide (BTS), a potent and specific inhibitor of fast muscle myosin-II, using small bundles of intact fibres or single fibres from rat foot muscle. BTS decreased tetanic tension reversibly in a concentration-dependent manner with half-maximal inhibition at similar to 2 mu M at 20 degrees C. The inhibition of tension with 10 mu M BTS was marked at the three temperatures examined (10, 20 and 30 degrees C), but greatest at 10 degrees C. BTS decreased active muscle stiffness to a lesser extent than tetanic tension indicating that not all of the tension inhibition was due to a reduced number of attached cross-bridges. BTS-induced inhibition of active tension was not accompanied by any change in the free myoplasmic Ca2+ transients. The potency and specificity of BTS make it a very suitable myosin inhibitor for intact mammalian fast muscle and should be a useful tool for the examination of outstanding questions in muscle contraction.
    Original languageEnglish
    Pages (from-to)135-141
    JournalJournal of Muscle Research and Cell Motility
    Volume26
    Issue number2/3
    DOIs
    Publication statusPublished - 2005

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