Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice

W.L.F. Lim, S.M. Lam, G. Shui, A. Mondal, D. Ong, X. Duan, R. Creegan, I.J. Martins, M.J. Sharman, K. Taddei, G. Verdile, M.R. Wenk, Ralph Martins

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    16 Citations (Scopus)

    Abstract

    Apolipoprotein E (ApoE) is important in facilitating the transport of lipids (cholesterol, phospholipids, and sulfatides) and plays a fundamental role in normal lipid metabolism. High cholesterol levels increases the risk of developing Alzheimer's disease. In this study, we investigated the effects of a high-fat high cholesterol (HFHC) diet on brain lipid profiles in 95 young and aged APOE e{open}3 and e{open}4 knock-in mice to determine whether diet leads to altered brain levels of a number of glycerophospholipids, sphingolipids, cholesterol precursors, cholesterol, cholesterol oxidation products, and cholesterol esters. The results in this study revealed significant changes in lipid levels. The HFHC-enriched diet influenced the levels of cholesterol esters. A sharp increase in cholesterol ester levels, particularly in the aged APOE e{open}4 diet-enriched group, might be suggestive of abnormal acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT) activity and/or levels. Age exerts appreciable effects on the brain lipidome, especially with regard to polar lipid species. © 2013 Elsevier Inc.
    Original languageEnglish
    Pages (from-to)2217-2224
    JournalNeurobiology of Aging
    Volume34
    Issue number9
    DOIs
    Publication statusPublished - 2013

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