Effect of P stoichiometry on the abundance of nitrogen-cycle genes in phosphorus-limited paddy soil

Xiaomeng Wei, Yajun Hu, Peiqin Peng, Zhenke Zhu, Cornelius Talade Atere, A.G. O’Donnell, Jinshui Wu, Tida Ge

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Previous studies have shown that phosphorus addition to P-limited soils increases gaseous N loss. A possible explanation for this phenomenon is element stoichiometry (specifically of C:N:P) modifying linked nutrient cycling, leading to enhanced nitrification and denitrification. In this study, we investigated how P stoichiometry influenced the dynamics of soil N-cycle functional genes. Rice seedlings were planted in P-poor soils and incubated with or without P application. Quantitative PCR was then applied to analyze the abundance of ammonia-oxidizing (amoA) and denitrifying (narG nirK, nirS, nosZ) genes in soil. P addition reduced bacterial amoA abundance but increased denitrifying gene abundance. We suggest this outcome is due to P-induced shifts in soil C:P and N:P ratios that limited ammonia oxidization while enhancing P availability for denitrification. Under P application, the rhizosphere effect raised ammonia-oxidizing bacterial abundance (amoA gene) and reduced nirK, nirS, and nosZ in rhizosphere soils. The change likely occurred through greater C input and O2 release from roots, thus altering C availability and redox conditions for microbes. Our results show that P application enhances gaseous N loss potential in paddy fields mainly through stimulating denitrifier growth. We conclude that nutrient availability and elemental stoichiometry are important in regulating microbial gene responses, thereby influencing key ecosystem processes such as denitrification. [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)767-776
Number of pages10
JournalBiology and Fertility of Soils
Volume53
Issue number7
DOIs
Publication statusPublished - 1 Oct 2017

Fingerprint

Nitrogen Cycle
nitrogen cycle
paddy soils
stoichiometry
Phosphorus
Ammonia
Soil
phosphorus
ammonia
Denitrification
gene
denitrification
Genes
soil
Rhizosphere
genes
rhizosphere
Microbial Genes
Nitrification
Food

Cite this

Wei, Xiaomeng ; Hu, Yajun ; Peng, Peiqin ; Zhu, Zhenke ; Atere, Cornelius Talade ; O’Donnell, A.G. ; Wu, Jinshui ; Ge, Tida. / Effect of P stoichiometry on the abundance of nitrogen-cycle genes in phosphorus-limited paddy soil. In: Biology and Fertility of Soils. 2017 ; Vol. 53, No. 7. pp. 767-776.
@article{c671d376d8d2473490a3b55aa66b5bc0,
title = "Effect of P stoichiometry on the abundance of nitrogen-cycle genes in phosphorus-limited paddy soil",
abstract = "Previous studies have shown that phosphorus addition to P-limited soils increases gaseous N loss. A possible explanation for this phenomenon is element stoichiometry (specifically of C:N:P) modifying linked nutrient cycling, leading to enhanced nitrification and denitrification. In this study, we investigated how P stoichiometry influenced the dynamics of soil N-cycle functional genes. Rice seedlings were planted in P-poor soils and incubated with or without P application. Quantitative PCR was then applied to analyze the abundance of ammonia-oxidizing (amoA) and denitrifying (narG nirK, nirS, nosZ) genes in soil. P addition reduced bacterial amoA abundance but increased denitrifying gene abundance. We suggest this outcome is due to P-induced shifts in soil C:P and N:P ratios that limited ammonia oxidization while enhancing P availability for denitrification. Under P application, the rhizosphere effect raised ammonia-oxidizing bacterial abundance (amoA gene) and reduced nirK, nirS, and nosZ in rhizosphere soils. The change likely occurred through greater C input and O2 release from roots, thus altering C availability and redox conditions for microbes. Our results show that P application enhances gaseous N loss potential in paddy fields mainly through stimulating denitrifier growth. We conclude that nutrient availability and elemental stoichiometry are important in regulating microbial gene responses, thereby influencing key ecosystem processes such as denitrification. [Figure not available: see fulltext.].",
keywords = "Ammonia oxidation, Denitrification, Functional genes, N cycling, Nutrient balance, Phosphorus",
author = "Xiaomeng Wei and Yajun Hu and Peiqin Peng and Zhenke Zhu and Atere, {Cornelius Talade} and A.G. O’Donnell and Jinshui Wu and Tida Ge",
year = "2017",
month = "10",
day = "1",
doi = "10.1007/s00374-017-1221-1",
language = "English",
volume = "53",
pages = "767--776",
journal = "Biology and Fertility of Soils",
issn = "0178-2762",
publisher = "Springer",
number = "7",

}

Effect of P stoichiometry on the abundance of nitrogen-cycle genes in phosphorus-limited paddy soil. / Wei, Xiaomeng; Hu, Yajun; Peng, Peiqin; Zhu, Zhenke; Atere, Cornelius Talade; O’Donnell, A.G.; Wu, Jinshui; Ge, Tida.

In: Biology and Fertility of Soils, Vol. 53, No. 7, 01.10.2017, p. 767-776.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of P stoichiometry on the abundance of nitrogen-cycle genes in phosphorus-limited paddy soil

AU - Wei, Xiaomeng

AU - Hu, Yajun

AU - Peng, Peiqin

AU - Zhu, Zhenke

AU - Atere, Cornelius Talade

AU - O’Donnell, A.G.

AU - Wu, Jinshui

AU - Ge, Tida

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Previous studies have shown that phosphorus addition to P-limited soils increases gaseous N loss. A possible explanation for this phenomenon is element stoichiometry (specifically of C:N:P) modifying linked nutrient cycling, leading to enhanced nitrification and denitrification. In this study, we investigated how P stoichiometry influenced the dynamics of soil N-cycle functional genes. Rice seedlings were planted in P-poor soils and incubated with or without P application. Quantitative PCR was then applied to analyze the abundance of ammonia-oxidizing (amoA) and denitrifying (narG nirK, nirS, nosZ) genes in soil. P addition reduced bacterial amoA abundance but increased denitrifying gene abundance. We suggest this outcome is due to P-induced shifts in soil C:P and N:P ratios that limited ammonia oxidization while enhancing P availability for denitrification. Under P application, the rhizosphere effect raised ammonia-oxidizing bacterial abundance (amoA gene) and reduced nirK, nirS, and nosZ in rhizosphere soils. The change likely occurred through greater C input and O2 release from roots, thus altering C availability and redox conditions for microbes. Our results show that P application enhances gaseous N loss potential in paddy fields mainly through stimulating denitrifier growth. We conclude that nutrient availability and elemental stoichiometry are important in regulating microbial gene responses, thereby influencing key ecosystem processes such as denitrification. [Figure not available: see fulltext.].

AB - Previous studies have shown that phosphorus addition to P-limited soils increases gaseous N loss. A possible explanation for this phenomenon is element stoichiometry (specifically of C:N:P) modifying linked nutrient cycling, leading to enhanced nitrification and denitrification. In this study, we investigated how P stoichiometry influenced the dynamics of soil N-cycle functional genes. Rice seedlings were planted in P-poor soils and incubated with or without P application. Quantitative PCR was then applied to analyze the abundance of ammonia-oxidizing (amoA) and denitrifying (narG nirK, nirS, nosZ) genes in soil. P addition reduced bacterial amoA abundance but increased denitrifying gene abundance. We suggest this outcome is due to P-induced shifts in soil C:P and N:P ratios that limited ammonia oxidization while enhancing P availability for denitrification. Under P application, the rhizosphere effect raised ammonia-oxidizing bacterial abundance (amoA gene) and reduced nirK, nirS, and nosZ in rhizosphere soils. The change likely occurred through greater C input and O2 release from roots, thus altering C availability and redox conditions for microbes. Our results show that P application enhances gaseous N loss potential in paddy fields mainly through stimulating denitrifier growth. We conclude that nutrient availability and elemental stoichiometry are important in regulating microbial gene responses, thereby influencing key ecosystem processes such as denitrification. [Figure not available: see fulltext.].

KW - Ammonia oxidation

KW - Denitrification

KW - Functional genes

KW - N cycling

KW - Nutrient balance

KW - Phosphorus

UR - http://www.scopus.com/inward/record.url?scp=85021809240&partnerID=8YFLogxK

U2 - 10.1007/s00374-017-1221-1

DO - 10.1007/s00374-017-1221-1

M3 - Article

VL - 53

SP - 767

EP - 776

JO - Biology and Fertility of Soils

JF - Biology and Fertility of Soils

SN - 0178-2762

IS - 7

ER -