Effect of high-intensity focused ultrasound on Enterococcus faecalis planktonic suspensions and biofilms

Kulsum Iqbal, Siew-Wan Ohl, Boo-Cheong Khoo, Jennifer Neo, Amr S Fawzy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study, the effect of high-intensity focused ultrasound (HIFU) on Enterococcus faecalis on both planktonic suspensions and biofilms was investigated. E. faecalis persist in secondary dental infections as biofilms. Glass-bottom Petri dishes with biofilms were centered at the focal point of the HIFU wave generated by a 250-kHz transducer. Specimens were subjected to HIFU exposure at different periods of 30, 60 and 120 s. The viable bacteria, removal effect and bacterial viability of biofilms attached to the Petri dish surface were studied by colony-forming units (CFUs), scanning electron microscopy and confocal microscopy, respectively. The removal and bactericidal effects of HIFU are dependent on the exposure time. A significant reduction in biofilm thickness and CFU was found with the increase in HIFU exposure. The removal or bactericidal effect of HIFU was more significant starting from 60 s of exposure. This study highlighted the potential application of HIFU as a novel method for root canal disinfection.

Original languageEnglish
Pages (from-to)825-33
Number of pages9
JournalUltrasound in Medicine & Biology
Volume39
Issue number5
DOIs
Publication statusPublished - May 2013
Externally publishedYes

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