Effect of changes in the surface chemistry and topography of poly(2-hydroxyethyl methacrylate) on the in vitro attachment of human corneal epithelial cells

Miriem Santander-Borrego, Traian V. Chirila, Audra M. A. Shadforth, Andrew K. Whittaker, Idriss Blakey

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The effects on cell adhesion induced by changes in the topography and chemistry of poly(2-hydroxyethyl methacrylate) hydrogel surfaces were investigated in vitro using the human corneal epithelial cell line, HCE-T. Poly(2-hydroxyethyl methacrylate) surfaces with a lotus-leaf-like topography and poly(2-hydroxyethyl methacrylate) surfaces with a flat topography, but functionalized with the cell-adhesive peptide sequence Arg–Gly–Asp, both enhanced attachment of HCE-T cells as compared to flat, non-functionalized poly(2-hydroxyethyl methacrylate) surfaces. However, the simultaneous existence on the same poly(2-hydroxyethyl methacrylate) surface of Arg–Gly–Asp motifs and of lotus-leaf-like topographical patterns led to an apparently antagonistic effect reflected in reduced cell attachment. The study provided additional evidence of the complexity of the cell–biomaterial interactions.
Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalJournal of Bioactive and Compatible Polymers
DOIs
Publication statusE-pub ahead of print - 20 Dec 2017

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