Effect of both ultraviolet B irradiation and histamine receptor function on allergic responses to an inhaled antigen

J.P. Mcglade, Shelley Gorman, J.C. Lenzo, J.W. Tan, T. Watanabe, John Finlay-Jones, Wayne Thomas, Prudence Hart

Research output: Contribution to journalArticle

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Abstract

Exposure of skin to UVB radiation (290-320 nm) modulates the immune system, with most studies showing a suppression of Th1-driven immune responses. This study investigated the effects of UVB on Th2-associated immune responses using a murine model of allergic respiratory inflammation. C57BL/6, histamine receptor-1 knockout (H1RKO), and histamine receptor-2 knockout (H2RKO) mice were exposed to a single 4 kJ/m(2) dose of UVB (twice a minimal edemal dose) on shaved dorsal skin 3 days before intranasal sensitization with papain, a cysteine protease homologue of the dust mite allergen Der p 1. HIRKO mice demonstrated enhanced papain-specific inflammatory responses in the lung-draining lymph nodes (LDLNs), whereas the responses of H2RKO mice closely mimicked those of C57BL/6 mice. UVB irradiation 3 days before sensitization reduced in vitro papain-specific proliferation of LDLN cells of C57BL/6 and HIRKO mice but not H2RKO mice 24 h after challenge. The regulatory effect of UVB was transferred by adoptive transfer of unfractionated LDLN cells from UVB-irradiated, papain-sensitized C57BL/6 and HIRKO donor mice in naive recipients of the corresponding strain that were subsequently sensitized and challenged with papain. Additionally, UVB exposure suppressed papain-induced IL-5 and IL-10 production in vitro by LDLN cells from HIRKO mice but not from C57BL/6 mice or H2RKO mice. The results of this study demonstrate systemic immuno-modulation of responses to intranasally delivered Ag by UVB irradiation and implicate a role for the H2 receptor in UV13-induced suppression of Ag-specific responses in the draining lymph nodes.
Original languageEnglish
Pages (from-to)2794-2802
JournalJournal of Immunology
Volume178
Issue number5
DOIs
Publication statusPublished - 2007

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Histamine Receptors
Papain
Antigens
Lymph Nodes
Lung
Inbred C57BL Mouse
Skin
Histamine H2 Receptors
Cysteine Proteases
Adoptive Transfer
Mites
Interleukin-5
Dust
Knockout Mice
Interleukin-10
Allergens
Immune System
Radiation
Inflammation

Cite this

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title = "Effect of both ultraviolet B irradiation and histamine receptor function on allergic responses to an inhaled antigen",
abstract = "Exposure of skin to UVB radiation (290-320 nm) modulates the immune system, with most studies showing a suppression of Th1-driven immune responses. This study investigated the effects of UVB on Th2-associated immune responses using a murine model of allergic respiratory inflammation. C57BL/6, histamine receptor-1 knockout (H1RKO), and histamine receptor-2 knockout (H2RKO) mice were exposed to a single 4 kJ/m(2) dose of UVB (twice a minimal edemal dose) on shaved dorsal skin 3 days before intranasal sensitization with papain, a cysteine protease homologue of the dust mite allergen Der p 1. HIRKO mice demonstrated enhanced papain-specific inflammatory responses in the lung-draining lymph nodes (LDLNs), whereas the responses of H2RKO mice closely mimicked those of C57BL/6 mice. UVB irradiation 3 days before sensitization reduced in vitro papain-specific proliferation of LDLN cells of C57BL/6 and HIRKO mice but not H2RKO mice 24 h after challenge. The regulatory effect of UVB was transferred by adoptive transfer of unfractionated LDLN cells from UVB-irradiated, papain-sensitized C57BL/6 and HIRKO donor mice in naive recipients of the corresponding strain that were subsequently sensitized and challenged with papain. Additionally, UVB exposure suppressed papain-induced IL-5 and IL-10 production in vitro by LDLN cells from HIRKO mice but not from C57BL/6 mice or H2RKO mice. The results of this study demonstrate systemic immuno-modulation of responses to intranasally delivered Ag by UVB irradiation and implicate a role for the H2 receptor in UV13-induced suppression of Ag-specific responses in the draining lymph nodes.",
author = "J.P. Mcglade and Shelley Gorman and J.C. Lenzo and J.W. Tan and T. Watanabe and John Finlay-Jones and Wayne Thomas and Prudence Hart",
year = "2007",
doi = "10.4049/jimmunol.178.5.2794",
language = "English",
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Effect of both ultraviolet B irradiation and histamine receptor function on allergic responses to an inhaled antigen. / Mcglade, J.P.; Gorman, Shelley; Lenzo, J.C.; Tan, J.W.; Watanabe, T.; Finlay-Jones, John; Thomas, Wayne; Hart, Prudence.

In: Journal of Immunology, Vol. 178, No. 5, 2007, p. 2794-2802.

Research output: Contribution to journalArticle

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T1 - Effect of both ultraviolet B irradiation and histamine receptor function on allergic responses to an inhaled antigen

AU - Mcglade, J.P.

AU - Gorman, Shelley

AU - Lenzo, J.C.

AU - Tan, J.W.

AU - Watanabe, T.

AU - Finlay-Jones, John

AU - Thomas, Wayne

AU - Hart, Prudence

PY - 2007

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N2 - Exposure of skin to UVB radiation (290-320 nm) modulates the immune system, with most studies showing a suppression of Th1-driven immune responses. This study investigated the effects of UVB on Th2-associated immune responses using a murine model of allergic respiratory inflammation. C57BL/6, histamine receptor-1 knockout (H1RKO), and histamine receptor-2 knockout (H2RKO) mice were exposed to a single 4 kJ/m(2) dose of UVB (twice a minimal edemal dose) on shaved dorsal skin 3 days before intranasal sensitization with papain, a cysteine protease homologue of the dust mite allergen Der p 1. HIRKO mice demonstrated enhanced papain-specific inflammatory responses in the lung-draining lymph nodes (LDLNs), whereas the responses of H2RKO mice closely mimicked those of C57BL/6 mice. UVB irradiation 3 days before sensitization reduced in vitro papain-specific proliferation of LDLN cells of C57BL/6 and HIRKO mice but not H2RKO mice 24 h after challenge. The regulatory effect of UVB was transferred by adoptive transfer of unfractionated LDLN cells from UVB-irradiated, papain-sensitized C57BL/6 and HIRKO donor mice in naive recipients of the corresponding strain that were subsequently sensitized and challenged with papain. Additionally, UVB exposure suppressed papain-induced IL-5 and IL-10 production in vitro by LDLN cells from HIRKO mice but not from C57BL/6 mice or H2RKO mice. The results of this study demonstrate systemic immuno-modulation of responses to intranasally delivered Ag by UVB irradiation and implicate a role for the H2 receptor in UV13-induced suppression of Ag-specific responses in the draining lymph nodes.

AB - Exposure of skin to UVB radiation (290-320 nm) modulates the immune system, with most studies showing a suppression of Th1-driven immune responses. This study investigated the effects of UVB on Th2-associated immune responses using a murine model of allergic respiratory inflammation. C57BL/6, histamine receptor-1 knockout (H1RKO), and histamine receptor-2 knockout (H2RKO) mice were exposed to a single 4 kJ/m(2) dose of UVB (twice a minimal edemal dose) on shaved dorsal skin 3 days before intranasal sensitization with papain, a cysteine protease homologue of the dust mite allergen Der p 1. HIRKO mice demonstrated enhanced papain-specific inflammatory responses in the lung-draining lymph nodes (LDLNs), whereas the responses of H2RKO mice closely mimicked those of C57BL/6 mice. UVB irradiation 3 days before sensitization reduced in vitro papain-specific proliferation of LDLN cells of C57BL/6 and HIRKO mice but not H2RKO mice 24 h after challenge. The regulatory effect of UVB was transferred by adoptive transfer of unfractionated LDLN cells from UVB-irradiated, papain-sensitized C57BL/6 and HIRKO donor mice in naive recipients of the corresponding strain that were subsequently sensitized and challenged with papain. Additionally, UVB exposure suppressed papain-induced IL-5 and IL-10 production in vitro by LDLN cells from HIRKO mice but not from C57BL/6 mice or H2RKO mice. The results of this study demonstrate systemic immuno-modulation of responses to intranasally delivered Ag by UVB irradiation and implicate a role for the H2 receptor in UV13-induced suppression of Ag-specific responses in the draining lymph nodes.

U2 - 10.4049/jimmunol.178.5.2794

DO - 10.4049/jimmunol.178.5.2794

M3 - Article

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JF - The Journal of Immunology

SN - 0022-1767

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