Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 2794-2802 |
| Journal | Journal of Immunology |
| Volume | 178 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2007 |
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Effect of both ultraviolet B irradiation and histamine receptor function on allergic responses to an inhaled antigen. / Mcglade, J.P.; Gorman, Shelley; Lenzo, J.C.; Tan, J.W.; Watanabe, T.; Finlay-Jones, John; Thomas, Wayne; Hart, Prudence.
In: Journal of Immunology, Vol. 178, No. 5, 2007, p. 2794-2802.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effect of both ultraviolet B irradiation and histamine receptor function on allergic responses to an inhaled antigen
AU - Mcglade, J.P.
AU - Gorman, Shelley
AU - Lenzo, J.C.
AU - Tan, J.W.
AU - Watanabe, T.
AU - Finlay-Jones, John
AU - Thomas, Wayne
AU - Hart, Prudence
PY - 2007
Y1 - 2007
N2 - Exposure of skin to UVB radiation (290-320 nm) modulates the immune system, with most studies showing a suppression of Th1-driven immune responses. This study investigated the effects of UVB on Th2-associated immune responses using a murine model of allergic respiratory inflammation. C57BL/6, histamine receptor-1 knockout (H1RKO), and histamine receptor-2 knockout (H2RKO) mice were exposed to a single 4 kJ/m(2) dose of UVB (twice a minimal edemal dose) on shaved dorsal skin 3 days before intranasal sensitization with papain, a cysteine protease homologue of the dust mite allergen Der p 1. HIRKO mice demonstrated enhanced papain-specific inflammatory responses in the lung-draining lymph nodes (LDLNs), whereas the responses of H2RKO mice closely mimicked those of C57BL/6 mice. UVB irradiation 3 days before sensitization reduced in vitro papain-specific proliferation of LDLN cells of C57BL/6 and HIRKO mice but not H2RKO mice 24 h after challenge. The regulatory effect of UVB was transferred by adoptive transfer of unfractionated LDLN cells from UVB-irradiated, papain-sensitized C57BL/6 and HIRKO donor mice in naive recipients of the corresponding strain that were subsequently sensitized and challenged with papain. Additionally, UVB exposure suppressed papain-induced IL-5 and IL-10 production in vitro by LDLN cells from HIRKO mice but not from C57BL/6 mice or H2RKO mice. The results of this study demonstrate systemic immuno-modulation of responses to intranasally delivered Ag by UVB irradiation and implicate a role for the H2 receptor in UV13-induced suppression of Ag-specific responses in the draining lymph nodes.
AB - Exposure of skin to UVB radiation (290-320 nm) modulates the immune system, with most studies showing a suppression of Th1-driven immune responses. This study investigated the effects of UVB on Th2-associated immune responses using a murine model of allergic respiratory inflammation. C57BL/6, histamine receptor-1 knockout (H1RKO), and histamine receptor-2 knockout (H2RKO) mice were exposed to a single 4 kJ/m(2) dose of UVB (twice a minimal edemal dose) on shaved dorsal skin 3 days before intranasal sensitization with papain, a cysteine protease homologue of the dust mite allergen Der p 1. HIRKO mice demonstrated enhanced papain-specific inflammatory responses in the lung-draining lymph nodes (LDLNs), whereas the responses of H2RKO mice closely mimicked those of C57BL/6 mice. UVB irradiation 3 days before sensitization reduced in vitro papain-specific proliferation of LDLN cells of C57BL/6 and HIRKO mice but not H2RKO mice 24 h after challenge. The regulatory effect of UVB was transferred by adoptive transfer of unfractionated LDLN cells from UVB-irradiated, papain-sensitized C57BL/6 and HIRKO donor mice in naive recipients of the corresponding strain that were subsequently sensitized and challenged with papain. Additionally, UVB exposure suppressed papain-induced IL-5 and IL-10 production in vitro by LDLN cells from HIRKO mice but not from C57BL/6 mice or H2RKO mice. The results of this study demonstrate systemic immuno-modulation of responses to intranasally delivered Ag by UVB irradiation and implicate a role for the H2 receptor in UV13-induced suppression of Ag-specific responses in the draining lymph nodes.
U2 - 10.4049/jimmunol.178.5.2794
DO - 10.4049/jimmunol.178.5.2794
M3 - Article
VL - 178
SP - 2794
EP - 2802
JO - The Journal of Immunology
JF - The Journal of Immunology
SN - 0022-1767
IS - 5
ER -