Effect of acute plasma volume expansion on peripheral arteriolar tone in healthy subjects

Alison Calver, J Collier, D Green, P Vallance

Research output: Contribution to journalArticle

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Abstract

1. Using venous occlusion plethysmography, we have investigated the forearm blood flow response in healthy subjects to the acute plasma volume expansion caused by a rapid intravenous infusion of saline. The contribution made to this response by nitric oxide has been investigated using local intra-arterial infusions of the nitric oxide synthase inhibitor NG-monomethyl-L-arginine. 2. The infusion of 1000 ml of saline over 25 min caused plasma volume to increase by about 7%, and resulted in a rise in forearm blood flow, with no change in arterial blood pressure. The onset of the blood flow response occurred within 10 min and blood flow remained elevated above baseline 20 min after the end of the saline infusion. 3. Local intra-arterial infusion of NG-monomethyl-L-arginine alone caused a reduction in forearm blood flow which was maximal at the end of the infusion and gradually recovered to baseline levels over 40 min. 4. When local intra-arterial infusion of NG-monomethyl-L-arginine was followed by plasma volume expansion, the calculated effect of NG-monomethyl-L-arginine was such as to abolish the vasodilator response to saline. 5. The effect of local intra-arterial infusion of NG-monomethyl-L-arginine on forearm blood flow was greater when the drug was given after volume expansion had occurred, than when it was given before the administration of saline. However, in control experiments the vasoconstrictor response to noradrenaline was also enhanced after the administration of the volume load in comparison with the response to noradrenaline given alone.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)541-7
Number of pages7
JournalClinical Science
Volume83
Issue number5
DOIs
Publication statusPublished - Nov 1992
Externally publishedYes

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Plasma Volume
omega-N-Methylarginine
Intra Arterial Infusions
Healthy Volunteers
Forearm
Norepinephrine
Plethysmography
Vasoconstrictor Agents
Vasodilator Agents
Intravenous Infusions
Nitric Oxide Synthase
Arterial Pressure
Nitric Oxide
Pharmaceutical Preparations

Cite this

Calver, Alison ; Collier, J ; Green, D ; Vallance, P. / Effect of acute plasma volume expansion on peripheral arteriolar tone in healthy subjects. In: Clinical Science. 1992 ; Vol. 83, No. 5. pp. 541-7.
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Effect of acute plasma volume expansion on peripheral arteriolar tone in healthy subjects. / Calver, Alison; Collier, J; Green, D; Vallance, P.

In: Clinical Science, Vol. 83, No. 5, 11.1992, p. 541-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of acute plasma volume expansion on peripheral arteriolar tone in healthy subjects

AU - Calver, Alison

AU - Collier, J

AU - Green, D

AU - Vallance, P

PY - 1992/11

Y1 - 1992/11

N2 - 1. Using venous occlusion plethysmography, we have investigated the forearm blood flow response in healthy subjects to the acute plasma volume expansion caused by a rapid intravenous infusion of saline. The contribution made to this response by nitric oxide has been investigated using local intra-arterial infusions of the nitric oxide synthase inhibitor NG-monomethyl-L-arginine. 2. The infusion of 1000 ml of saline over 25 min caused plasma volume to increase by about 7%, and resulted in a rise in forearm blood flow, with no change in arterial blood pressure. The onset of the blood flow response occurred within 10 min and blood flow remained elevated above baseline 20 min after the end of the saline infusion. 3. Local intra-arterial infusion of NG-monomethyl-L-arginine alone caused a reduction in forearm blood flow which was maximal at the end of the infusion and gradually recovered to baseline levels over 40 min. 4. When local intra-arterial infusion of NG-monomethyl-L-arginine was followed by plasma volume expansion, the calculated effect of NG-monomethyl-L-arginine was such as to abolish the vasodilator response to saline. 5. The effect of local intra-arterial infusion of NG-monomethyl-L-arginine on forearm blood flow was greater when the drug was given after volume expansion had occurred, than when it was given before the administration of saline. However, in control experiments the vasoconstrictor response to noradrenaline was also enhanced after the administration of the volume load in comparison with the response to noradrenaline given alone.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - 1. Using venous occlusion plethysmography, we have investigated the forearm blood flow response in healthy subjects to the acute plasma volume expansion caused by a rapid intravenous infusion of saline. The contribution made to this response by nitric oxide has been investigated using local intra-arterial infusions of the nitric oxide synthase inhibitor NG-monomethyl-L-arginine. 2. The infusion of 1000 ml of saline over 25 min caused plasma volume to increase by about 7%, and resulted in a rise in forearm blood flow, with no change in arterial blood pressure. The onset of the blood flow response occurred within 10 min and blood flow remained elevated above baseline 20 min after the end of the saline infusion. 3. Local intra-arterial infusion of NG-monomethyl-L-arginine alone caused a reduction in forearm blood flow which was maximal at the end of the infusion and gradually recovered to baseline levels over 40 min. 4. When local intra-arterial infusion of NG-monomethyl-L-arginine was followed by plasma volume expansion, the calculated effect of NG-monomethyl-L-arginine was such as to abolish the vasodilator response to saline. 5. The effect of local intra-arterial infusion of NG-monomethyl-L-arginine on forearm blood flow was greater when the drug was given after volume expansion had occurred, than when it was given before the administration of saline. However, in control experiments the vasoconstrictor response to noradrenaline was also enhanced after the administration of the volume load in comparison with the response to noradrenaline given alone.(ABSTRACT TRUNCATED AT 250 WORDS)

KW - Arginine/analogs & derivatives

KW - Arterioles/physiology

KW - Forearm/blood supply

KW - Humans

KW - Norepinephrine/pharmacology

KW - Plasma Volume/physiology

KW - Plethysmography

KW - Western Australia

KW - Sodium Chloride/pharmacology

KW - Vasodilation/drug effects

KW - omega-N-Methylarginine

U2 - 10.1042/cs0830541

DO - 10.1042/cs0830541

M3 - Article

VL - 83

SP - 541

EP - 547

JO - Clinical Science

JF - Clinical Science

SN - 0143-5221

IS - 5

ER -