Effect of 5-bromodeoxyuridine on the appearance of the liver isoenzyme of pyruvate kinase

R. Scott, G. C.T. Yeoh

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Hepatocyte cultures derived from 15-day foetal rats produce the liver form of pyruvate kinase (EC 2.7.1.40) only after 3 days of culture. The appearance of the liver form of the enzyme can be blocked by the addition of 5-bromodeoxyuridine on day 2 of culture, but not by addition on day 3 of culture. The reversibility of the action of 5-bromodeoxyuridine was shown when the inhibitor was added on day 2 and removed on day 4. By day 6 of culture the liver form of pyruvate kinase was detectable. The specificity of the action of 5-bromodeoxyuridine was monitored by following changes in the closely related embryonic form of the enzyme as a control. This was unaltered by the inhibitor.

    Original languageEnglish
    Pages (from-to)865-868
    Number of pages4
    JournalBiochemical Journal
    Volume220
    Issue number3
    DOIs
    Publication statusPublished - 1 Jan 1984

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