Effect of ω-3 fatty acid ethyl esters on apolipoprotein B-48 kinetics in obese subjects on a weight-loss diet: a new tracer kinetic study in the postprandial state

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    Abstract

    Context: Dysregulated chylomicron metabolism may account for hypertriglyceridemia and increased risk of cardiovascular disease in obese subjects. Supplementation with ω-3 fatty acid ethyl ester (FAEE) decreases plasma triglyceride. However, its effect on postprandial chylomicron metabolism in obese subjects on a weight-loss diet has not yet been investigated. Objective: We aimed to examine the effect of ω-3 FAEE supplementation on apolipoprotein (apo) B-48 kinetics in obese subjects on a weight-loss diet. Design, Setting, and Patients:Wecarried out a 12-week, randomized trial of a hypocaloric diet plus 4 g/dω-3 FAEE supplementation (46% eicosapentaenoic acid and 38% docosahexaenoic acid) (n= 13) compared with a hypocaloric diet alone (n = 12) on postprandial apoB-48 kinetics in obese subjects after ingestion of an oral load. The apoB-48 kinetics were determined using stable isotope tracer kinetics and multicompartmental modeling. Outcomes Measures:Weevaluated plasma totalandincremental apoB-48 0- to 10-hour area under the curves (AUCs) as well as apoB-48 secretion and fractional catabolic rate. Results: Weight loss with or withoutω-3 FAEE supplementation significantly reduced body weight, total fat mass, homeostasis model assessment score, fasting triglyceride concentration, postprandial triglycerideAUC,andincreased plasma high-density lipoprotein cholesterol concentration (P<.05 in all). Compared with weight loss alone, weight loss plus ω-3 FAEE significantly (all P <.05) decreased fasting triglyceride (-11%), apoB-48 (-36%) concentrations, postprandial triglyceride (-21%), and apoB-48 (-22%) total AUCs, as well as incremental postprandial triglyceride AUCs (-32%). The ω-3 FAEE also significantly decreased apoB-48 secretion in the basal state, without a significant effect during the postprandial period (3-6 hours). The fractional catabolic rate of apoB-48 increased with both interventions with no significant independent effect of ω-3 FAEE supplementation. Conclusion: Addition ofω-3FAEEsupplementation to amoderateweight-loss diet in obese subjects can significantly improve chylomicron metabolism by independently decreasing the secretion of apoB-48. Copyright © 2014 by the Endocrine Society.
    Original languageEnglish
    Pages (from-to)E1427-E1435
    JournalJournal of Clinical Endocrinology and Metabolism
    Volume99
    Issue number8
    Early online date27 Feb 2014
    DOIs
    Publication statusPublished - Aug 2014

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    Apolipoprotein B-48
    Reducing Diet
    Nutrition
    Esters
    Fatty Acids
    Kinetics
    Triglycerides
    Chylomicrons
    Metabolism
    Area Under Curve
    Weight Loss
    Diet
    Plasmas
    Fasting
    Postprandial Period
    Eicosapentaenoic Acid
    Docosahexaenoic Acids
    Hypertriglyceridemia
    Isotopes
    HDL Cholesterol

    Cite this

    @article{461b610c308543c2af1bf9409b4748ea,
    title = "Effect of ω-3 fatty acid ethyl esters on apolipoprotein B-48 kinetics in obese subjects on a weight-loss diet: a new tracer kinetic study in the postprandial state",
    abstract = "Context: Dysregulated chylomicron metabolism may account for hypertriglyceridemia and increased risk of cardiovascular disease in obese subjects. Supplementation with ω-3 fatty acid ethyl ester (FAEE) decreases plasma triglyceride. However, its effect on postprandial chylomicron metabolism in obese subjects on a weight-loss diet has not yet been investigated. Objective: We aimed to examine the effect of ω-3 FAEE supplementation on apolipoprotein (apo) B-48 kinetics in obese subjects on a weight-loss diet. Design, Setting, and Patients:Wecarried out a 12-week, randomized trial of a hypocaloric diet plus 4 g/dω-3 FAEE supplementation (46{\%} eicosapentaenoic acid and 38{\%} docosahexaenoic acid) (n= 13) compared with a hypocaloric diet alone (n = 12) on postprandial apoB-48 kinetics in obese subjects after ingestion of an oral load. The apoB-48 kinetics were determined using stable isotope tracer kinetics and multicompartmental modeling. Outcomes Measures:Weevaluated plasma totalandincremental apoB-48 0- to 10-hour area under the curves (AUCs) as well as apoB-48 secretion and fractional catabolic rate. Results: Weight loss with or withoutω-3 FAEE supplementation significantly reduced body weight, total fat mass, homeostasis model assessment score, fasting triglyceride concentration, postprandial triglycerideAUC,andincreased plasma high-density lipoprotein cholesterol concentration (P<.05 in all). Compared with weight loss alone, weight loss plus ω-3 FAEE significantly (all P <.05) decreased fasting triglyceride (-11{\%}), apoB-48 (-36{\%}) concentrations, postprandial triglyceride (-21{\%}), and apoB-48 (-22{\%}) total AUCs, as well as incremental postprandial triglyceride AUCs (-32{\%}). The ω-3 FAEE also significantly decreased apoB-48 secretion in the basal state, without a significant effect during the postprandial period (3-6 hours). The fractional catabolic rate of apoB-48 increased with both interventions with no significant independent effect of ω-3 FAEE supplementation. Conclusion: Addition ofω-3FAEEsupplementation to amoderateweight-loss diet in obese subjects can significantly improve chylomicron metabolism by independently decreasing the secretion of apoB-48. Copyright {\circledC} 2014 by the Endocrine Society.",
    author = "A.T.Y. Wong and Dick Chan and Hugh Barrett and Leon Adams and Gerald Watts",
    year = "2014",
    month = "8",
    doi = "10.1210/jc.2013-4037",
    language = "English",
    volume = "99",
    pages = "E1427--E1435",
    journal = "Journal of Endocrinology & Metabolism",
    issn = "0021-972X",
    publisher = "ENDOCRINE SOC",
    number = "8",

    }

    TY - JOUR

    T1 - Effect of ω-3 fatty acid ethyl esters on apolipoprotein B-48 kinetics in obese subjects on a weight-loss diet: a new tracer kinetic study in the postprandial state

    AU - Wong, A.T.Y.

    AU - Chan, Dick

    AU - Barrett, Hugh

    AU - Adams, Leon

    AU - Watts, Gerald

    PY - 2014/8

    Y1 - 2014/8

    N2 - Context: Dysregulated chylomicron metabolism may account for hypertriglyceridemia and increased risk of cardiovascular disease in obese subjects. Supplementation with ω-3 fatty acid ethyl ester (FAEE) decreases plasma triglyceride. However, its effect on postprandial chylomicron metabolism in obese subjects on a weight-loss diet has not yet been investigated. Objective: We aimed to examine the effect of ω-3 FAEE supplementation on apolipoprotein (apo) B-48 kinetics in obese subjects on a weight-loss diet. Design, Setting, and Patients:Wecarried out a 12-week, randomized trial of a hypocaloric diet plus 4 g/dω-3 FAEE supplementation (46% eicosapentaenoic acid and 38% docosahexaenoic acid) (n= 13) compared with a hypocaloric diet alone (n = 12) on postprandial apoB-48 kinetics in obese subjects after ingestion of an oral load. The apoB-48 kinetics were determined using stable isotope tracer kinetics and multicompartmental modeling. Outcomes Measures:Weevaluated plasma totalandincremental apoB-48 0- to 10-hour area under the curves (AUCs) as well as apoB-48 secretion and fractional catabolic rate. Results: Weight loss with or withoutω-3 FAEE supplementation significantly reduced body weight, total fat mass, homeostasis model assessment score, fasting triglyceride concentration, postprandial triglycerideAUC,andincreased plasma high-density lipoprotein cholesterol concentration (P<.05 in all). Compared with weight loss alone, weight loss plus ω-3 FAEE significantly (all P <.05) decreased fasting triglyceride (-11%), apoB-48 (-36%) concentrations, postprandial triglyceride (-21%), and apoB-48 (-22%) total AUCs, as well as incremental postprandial triglyceride AUCs (-32%). The ω-3 FAEE also significantly decreased apoB-48 secretion in the basal state, without a significant effect during the postprandial period (3-6 hours). The fractional catabolic rate of apoB-48 increased with both interventions with no significant independent effect of ω-3 FAEE supplementation. Conclusion: Addition ofω-3FAEEsupplementation to amoderateweight-loss diet in obese subjects can significantly improve chylomicron metabolism by independently decreasing the secretion of apoB-48. Copyright © 2014 by the Endocrine Society.

    AB - Context: Dysregulated chylomicron metabolism may account for hypertriglyceridemia and increased risk of cardiovascular disease in obese subjects. Supplementation with ω-3 fatty acid ethyl ester (FAEE) decreases plasma triglyceride. However, its effect on postprandial chylomicron metabolism in obese subjects on a weight-loss diet has not yet been investigated. Objective: We aimed to examine the effect of ω-3 FAEE supplementation on apolipoprotein (apo) B-48 kinetics in obese subjects on a weight-loss diet. Design, Setting, and Patients:Wecarried out a 12-week, randomized trial of a hypocaloric diet plus 4 g/dω-3 FAEE supplementation (46% eicosapentaenoic acid and 38% docosahexaenoic acid) (n= 13) compared with a hypocaloric diet alone (n = 12) on postprandial apoB-48 kinetics in obese subjects after ingestion of an oral load. The apoB-48 kinetics were determined using stable isotope tracer kinetics and multicompartmental modeling. Outcomes Measures:Weevaluated plasma totalandincremental apoB-48 0- to 10-hour area under the curves (AUCs) as well as apoB-48 secretion and fractional catabolic rate. Results: Weight loss with or withoutω-3 FAEE supplementation significantly reduced body weight, total fat mass, homeostasis model assessment score, fasting triglyceride concentration, postprandial triglycerideAUC,andincreased plasma high-density lipoprotein cholesterol concentration (P<.05 in all). Compared with weight loss alone, weight loss plus ω-3 FAEE significantly (all P <.05) decreased fasting triglyceride (-11%), apoB-48 (-36%) concentrations, postprandial triglyceride (-21%), and apoB-48 (-22%) total AUCs, as well as incremental postprandial triglyceride AUCs (-32%). The ω-3 FAEE also significantly decreased apoB-48 secretion in the basal state, without a significant effect during the postprandial period (3-6 hours). The fractional catabolic rate of apoB-48 increased with both interventions with no significant independent effect of ω-3 FAEE supplementation. Conclusion: Addition ofω-3FAEEsupplementation to amoderateweight-loss diet in obese subjects can significantly improve chylomicron metabolism by independently decreasing the secretion of apoB-48. Copyright © 2014 by the Endocrine Society.

    U2 - 10.1210/jc.2013-4037

    DO - 10.1210/jc.2013-4037

    M3 - Article

    VL - 99

    SP - E1427-E1435

    JO - Journal of Endocrinology & Metabolism

    JF - Journal of Endocrinology & Metabolism

    SN - 0021-972X

    IS - 8

    ER -