Dysregulation of innate immunity in ulcerative colitis patients who fail anti-Tumor necrosis factor therapy Basic Study

Angela Baird, D. Mallon, G. Radford-Smith, J. Boyer, T. Piche, Susan Prescott, Ian Lawrance, M.K. Tulic

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

© 2016 Baishideng Publishing Group Inc. All rights reserved.To study the innate immune function in ulcerative AIM To study the innate immune function in ulcerative Submit a Manuscript: http://www.wjgnet.com/esps/ Help Desk: http://www.wjgnet.com/esps/helpdesk.aspx DOI: 10.3748/wjg.v22.i41.9104 WJG|www.wjgnet.com 9104 November 7, 2016|Volume 22|Issue 41| World J Gastroenterol 2016 November 7; 22(41): 9104-9116 ISSN 1007-9327 (print) ISSN 2219-2840 (online). ORIGINAL ARTICLE Dysregulation of innate immunity in ulcerative colitis patients who fail anti-Tumor necrosis factor therapy Basic Study Angela C Baird, Dominic Mallon, Graham Radford-Smith, Julien Boyer, Thierry Piche, Susan L Prescott, Ian C Lawrance, Meri K Tulic colitis (UC) patients who fail to respond to anti-Tumor necrosis factor (TNF) therapy. METHODS Effects of anti-TNF therapy, inflammation and medications on innate immune function were assessed by measuring peripheral blood mononuclear cell (PBMC) cytokine expression from 18 inflammatory bowel disease patients pre-And 3 mo post-Anti-TNF therapy. Toll-like receptor (TLR) expression and cytokine production post TLR stimulation was assessed in UC "responders" (n = 12) and "non-responders" (n = 12) and compared to healthy controls (n = 12). Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) levels were measured in blood to assess disease severity/activity and inflammation. Pro-inflammatory (TNF, IL-1β, IL-6), immuno-regulatory (IL-10), Th1 (IL-12, IFNγ) and Th2 (IL-9, IL-13, IL-17A) cytokine expression was measured with enzyme-linked immunosorbent assay while TLR cellular composition and intracellular signalling was assessed with FACS. RESULTS Prior to anti-TNF therapy, responders and nonresponders had similar level of disease severity and activity. PBMC's ability to respond to TLR stimulation was not affected by TNF therapy, patient's severity of the disease and inflammation or their medication use. At baseline, non-responders had elevated innate but not adaptive immune responses compared to responders (P <0.05). Following TLR stimulation, nonresponders had consistently reduced innate cytokine responses to all TLRs compared to healthy controls (P <0.01) and diminished TNF (P <0.001) and IL- 1b (P <0.01) production compared to responders. This innate immune dysfunction was associated with reduced number of circulating plasmacytoid dendritic cells (pDCs) (P <0.01) but increased number of CD4+ regulatory T cells (Tregs) (P = 0.03) as well as intracellular accumulation of IRAK4 in non-responders following TLR-2, -4 and -7 activation (P <0.001). CONCLUSION Reduced innate immunity in non-responders may explain reduced efficacy to anti-TNF therapy. These serological markers may prove useful in predicting the outcome of costly anti-TNF therapy.
Original languageEnglish
Pages (from-to)9104-9116
Number of pages13
JournalWorld Journal of Gastroenterology
Volume22
Issue number41
DOIs
Publication statusPublished - 2016

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