@article{2854b8669d624e0f980fe671a205fff9,
title = "Dynamic dentin: A quantitative microscopic assessment of age and spatial changes to matrix architecture, peritubular dentin, and collagens types I and III",
abstract = "To investigate age and site-related changes to human dentin collagen, sound human teeth collected from donors aged 13–29 (young) and 50–74 (aged) years (n = 9/group) were cut to shallow and deep sites. Dentin collagen orientation and fibril bundling was investigated using the Picrosirius Red (PSR) stain observed under cross-polarized light microscopy (Pol), and collagen distribution was investigated using Confocal Laser Scanning Microscopy (CLSM). Collagen types III to I distribution in peritubular dentin (PTD) was revealed using Herovici stain and brightfield microscopy. Image analysis software and linear mixed modelling quantified outcomes. In situ dentin collagen was observed using Xenon Plasma Focussed Ion Beam Scanning Electron Microscopy (Xe PFIB-SEM). The PSR-Pol analysis revealed less coherently aligned and more bundled collagen fibrils in aged dentin (P = 0.005). Deep inner dentin collagen in both groups were less coherently aligned with reduced bundling. Regardless of age, CLSM showed collagen distribution remained stable; and more collagen type III was detectable in PTD located in inner dentin (Young: P = 0.006; Aged: P = 0.008). Observations following Xe PFIB-SEM cross-sectioning showed apatite-like deposits surrounding large intratubular collagen fibers, and evidence of smaller intertubular dentin collagen fibrils in situ. In conclusion, aging changes collagen network architecture, but not distribution or content.",
keywords = "Aging, Collagen, Dentin, Herovici, Picrosirius red, SEM",
author = "Weerakoon, {Arosha T.} and Nicholas Condon and Cox, {Thomas R.} and Christopher Sexton and Crystal Cooper and Meyers, {Ian A.} and David Thomson and Ford, {Pauline J.} and Sandrine Roy and Symons, {Anne L.}",
note = "Funding Information: We acknowledge Colgate Palmolive, Australia and the University of Queensland, School of Dentistry Post-Graduate Research Fund for supporting the cost of the project. The authors would also like to thank Drs Jim McGowan and Ian Young, and staff from Maxillo-facial & Implant Centre, Birtinya in Queensland, Australia and Dr Leesa Auckett for assisting with tooth collection; and Dr Rick and Mrs Robinson as well as staff, particularly Ms Julie Webb, at Cooroy Dental Surgery for assisting with tooth collection and providing the facilities to prepare the samples for this project. The authors acknowledge the facilities and the scientific and technical assistance from the Microscopy Australia linked lab at the Central Analytical Research Facility (CARF), and the technical assistance from Ms Miranda Kay and Mrs Felicity Lawrence from CARF, Queensland University of Technology, Brisbane, Australia. We also acknowledge the histology facilities at Queensland Institute for Medical Research, and the technical assistance from Mr Ali Ju and Mr Justin Large from Translational Research Institute Australia, Brisbane. Thank you to the design team from BioRender.Com for their generous feedback that enhanced the quality of the figures and graphical abstract that was created with the program. Funding Information: We acknowledge Colgate Palmolive, Australia and the University of Queensland, School of Dentistry Post-Graduate Research Fund for supporting the cost of the project. The authors would also like to thank Drs Jim McGowan and Ian Young, and staff from Maxillo-facial & Implant Centre, Birtinya in Queensland, Australia and Dr Leesa Auckett for assisting with tooth collection; and Dr Rick and Mrs Robinson as well as staff, particularly Ms Julie Webb, at Cooroy Dental Surgery for assisting with tooth collection and providing the facilities to prepare the samples for this project. The authors acknowledge the facilities and the scientific and technical assistance from the Microscopy Australia linked lab at the Central Analytical Research Facility (CARF), and the technical assistance from Ms Miranda Kay and Mrs Felicity Lawrence from CARF, Queensland University of Technology, Brisbane, Australia. We also acknowledge the histology facilities at Queensland Institute for Medical Research, and the technical assistance from Mr Ali Ju and Mr Justin Large from Translational Research Institute Australia, Brisbane. Thank you to the design team from BioRender.Com for their generous feedback that enhanced the quality of the figures and graphical abstract that was created with the program. Ethics Approval, The University of Queensland's Institutional Human Research Ethics Approval Committee (Approval Number 2019000532). Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",
year = "2022",
month = dec,
doi = "10.1016/j.jsb.2022.107899",
language = "English",
volume = "214",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press",
number = "4",
}