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Abstract
Copyright © 2016, American Society for Microbiology. All Rights Reserved.
We have developed a specific Haemophilus influenzae quantitative PCR (qPCR) that also identifies fucose-negative and protein D-negative strains. Analysis of 100 H. influenzae isolates, 28 Haemophilus haemolyticus isolates, and 14 other bacterial species revealed 100% sensitivity (95% confidence interval [CI], 96% to 100%) and 100% specificity (95% CI, 92% to 100%) for this assay. The evaluation of 80 clinical specimens demonstrated a strong correlation between semiquantitative culture and the qPCR (P< 0.001).
Original language | English |
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Pages (from-to) | 2380-2383 |
Number of pages | 4 |
Journal | Journal of Clinical Microbiology |
Volume | 54 |
Issue number | 9 |
DOIs | |
Publication status | Published - Sept 2016 |
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Dive into the research topics of 'Duplex quantitative PCR assay for detection of haemophilus influenzae that distinguishes fucose-and protein d-negative strains'. Together they form a unique fingerprint.Projects
- 2 Finished
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Evaluation of Haemophilus haemolyticus as a preventative therapy for NTHi disease
Kirkham, L.-A. (Investigator 01), Thornton, R. (Investigator 02), Bellgard, M. (Investigator 03) & Richmond, P. (Investigator 04)
NHMRC National Health and Medical Research Council
1/01/15 → 31/12/18
Project: Research
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Understanding respiratory infections to improve vaccines
Kirkham, L.-A. (Investigator 01)
NHMRC National Health and Medical Research Council
1/01/14 → 31/08/18
Project: Research