Duplex quantitative PCR assay for detection of haemophilus influenzae that distinguishes fucose-and protein d-negative strains

Camilla De Gier, Janessa L. Pickering, Peter C. Richmond, Ruth B. Thornton, Lea-Ann S. Kirkham

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    Abstract

    Copyright © 2016, American Society for Microbiology. All Rights Reserved.

    We have developed a specific Haemophilus influenzae quantitative PCR (qPCR) that also identifies fucose-negative and protein D-negative strains. Analysis of 100 H. influenzae isolates, 28 Haemophilus haemolyticus isolates, and 14 other bacterial species revealed 100% sensitivity (95% confidence interval [CI], 96% to 100%) and 100% specificity (95% CI, 92% to 100%) for this assay. The evaluation of 80 clinical specimens demonstrated a strong correlation between semiquantitative culture and the qPCR (P< 0.001).

    Original languageEnglish
    Pages (from-to)2380-2383
    JournalJournal of Clinical Microbiology
    Volume54
    Issue number9
    DOIs
    Publication statusPublished - Sep 2016

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