DNA ploidy variation and distribution in the Lepidosperma costale complex (Cyperaceae): Implications for conservation and restoration in a biodiversity hotspot

Mark J. Wallace, Lydia K. Guja, Marie A. Jouault, Kathy A. Fuller, Russell L. Barrett, Siegfried L. Krauss, Matthew D. Barrett

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Intraspecific ploidy variation is an important component of angiosperm biodiversity; however, this variation is rarely considered in conservation programs. This is of particular concern when conservation activities include augmentation, reintroduction or ecological restoration because there are potentially negative consequences when ploidy variants are unintentionally mixed within populations. We surveyed regional ploidy variation in the Lepidosperma costale Nees species complex (Schoeneae: Cyperaceae) in the South West Australian Floristic Region, an international biodiversity hotspot. Several L. costale sensu lato populations are threatened by iron-ore extraction, including the rare L. gibsonii R.L.Barrett, and these populations are the subject of ecological restoration programs. The DNA ploidy of 2384 individuals from 28 populations across the range of the species complex was determined and four DNA ploidy levels were discovered, namely, diploid, triploid, tetraploid and pentaploid. Diploids and tetraploids were the most common cytotypes and were largely geographically segregated, even at an exhaustively studied contact zone. Triploids were found at a low frequency in two populations. The rarity of triploids suggests substantial interploidy sterility, and that mixing of ploidy variants should, therefore, be avoided when restoring L. costale s.l. populations. These data provide a guide for L. costale s.l. germplasm collection and suggest that polyploidy may be an important driver of diversification in these sedges.

Original languageEnglish
Pages (from-to)120-127
Number of pages8
JournalAustralian Journal of Botany
Volume65
Issue number2
DOIs
Publication statusPublished - 2017

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ploidy
Cyperaceae
biodiversity
DNA
triploidy
ecological restoration
species complex
tetraploidy
diploidy
polyploidy
cytotypes
sterility
contact zone
conservation programs
germplasm
reintroduction
rarity
germplasm conservation
iron ore
angiosperm

Cite this

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title = "DNA ploidy variation and distribution in the Lepidosperma costale complex (Cyperaceae): Implications for conservation and restoration in a biodiversity hotspot",
abstract = "Intraspecific ploidy variation is an important component of angiosperm biodiversity; however, this variation is rarely considered in conservation programs. This is of particular concern when conservation activities include augmentation, reintroduction or ecological restoration because there are potentially negative consequences when ploidy variants are unintentionally mixed within populations. We surveyed regional ploidy variation in the Lepidosperma costale Nees species complex (Schoeneae: Cyperaceae) in the South West Australian Floristic Region, an international biodiversity hotspot. Several L. costale sensu lato populations are threatened by iron-ore extraction, including the rare L. gibsonii R.L.Barrett, and these populations are the subject of ecological restoration programs. The DNA ploidy of 2384 individuals from 28 populations across the range of the species complex was determined and four DNA ploidy levels were discovered, namely, diploid, triploid, tetraploid and pentaploid. Diploids and tetraploids were the most common cytotypes and were largely geographically segregated, even at an exhaustively studied contact zone. Triploids were found at a low frequency in two populations. The rarity of triploids suggests substantial interploidy sterility, and that mixing of ploidy variants should, therefore, be avoided when restoring L. costale s.l. populations. These data provide a guide for L. costale s.l. germplasm collection and suggest that polyploidy may be an important driver of diversification in these sedges.",
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DNA ploidy variation and distribution in the Lepidosperma costale complex (Cyperaceae) : Implications for conservation and restoration in a biodiversity hotspot. / Wallace, Mark J.; Guja, Lydia K.; Jouault, Marie A.; Fuller, Kathy A.; Barrett, Russell L.; Krauss, Siegfried L.; Barrett, Matthew D.

In: Australian Journal of Botany, Vol. 65, No. 2, 2017, p. 120-127.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DNA ploidy variation and distribution in the Lepidosperma costale complex (Cyperaceae)

T2 - Implications for conservation and restoration in a biodiversity hotspot

AU - Wallace, Mark J.

AU - Guja, Lydia K.

AU - Jouault, Marie A.

AU - Fuller, Kathy A.

AU - Barrett, Russell L.

AU - Krauss, Siegfried L.

AU - Barrett, Matthew D.

PY - 2017

Y1 - 2017

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KW - contact zone

KW - cytotype

KW - flow cytometry

KW - genome size

KW - granite

KW - ironstone

KW - polyploid.

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