DNA G-Quadruplex and i-Motif Structure Formation Is Interdependent in Human Cells

Jessica King; Kelly Irving; Cameron W. Evans; Rupesh Chikhale; Rouven Becker; Christopher  Morris; Cristian  Peña Martinez; Peter  Schofield; Daniel  Christ; Laurence Hurley; Zoë  Waller; Killugudi Swaminatha Iyer; Nicole Smith

doi:10.1021/jacs.0c11708

DNA G-Quadruplex and i-Motif Structure Formation Is Interdependent in Human Cells

Jessica King, Kelly Irving, Cameron W. Evans, Rupesh Chikhale, Rouven Becker, Christopher Morris, Cristian Peña Martinez, Peter Schofield, Daniel Christ, Laurence Hurley, Zoë Waller, Killugudi Swaminatha Iyer, Nicole Smith

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

Guanine- and cytosine-rich nucleic acid sequences have the potential to form secondary structures such as G-quadruplexes and i-motifs, respectively. We show that stabilization of G-quadruplexes using small molecules destabilizes the i-motifs, and vice versa, indicating these gene regulatory controllers are interdependent in human cells. This has important implications as these structures are predominately considered as isolated structural targets for therapy, but their interdependency highlights the interplay of both structures as an important gene regulatory switch.

Original language	English
Pages (from-to)	20600-20604
Number of pages	5
Journal	Journal of the Amercian Chemical Society
Volume	142
Issue number	49
DOIs	https://doi.org/10.1021/jacs.0c11708
Publication status	Published - 9 Dec 2020

Access to Document

10.1021/jacs.0c11708

https://ueaeprints.uea.ac.uk/id/eprint/77847

69 Citations
1 Doctoral Thesis

Characterisation of endogenous i-motif and G-quadruplex DNA structures in human chromatin and their relationship with cancer-associated genomic landscapes
Irving, K., 2023, (Unpublished)
Research output: Thesis › Doctoral Thesis

File
224 Downloads (Pure)

Cite this

@article{26403e6a6f364b9c99de29210c68e1ec,

title = "DNA G-Quadruplex and i-Motif Structure Formation Is Interdependent in Human Cells",

abstract = "Guanine- and cytosine-rich nucleic acid sequences have the potential to form secondary structures such as G-quadruplexes and i-motifs, respectively. We show that stabilization of G-quadruplexes using small molecules destabilizes the i-motifs, and vice versa, indicating these gene regulatory controllers are interdependent in human cells. This has important implications as these structures are predominately considered as isolated structural targets for therapy, but their interdependency highlights the interplay of both structures as an important gene regulatory switch.",

author = "Jessica King and Kelly Irving and Evans, {Cameron W.} and Rupesh Chikhale and Rouven Becker and Christopher Morris and {Pe{\~n}a Martinez}, Cristian and Peter Schofield and Daniel Christ and Laurence Hurley and Zo{\"e} Waller and {Swaminatha Iyer}, Killugudi and Nicole Smith",

year = "2020",

month = dec,

day = "9",

doi = "10.1021/jacs.0c11708",

language = "English",

volume = "142",

pages = "20600--20604",

journal = "Journal of the Amercian Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "49",

}

TY - JOUR

T1 - DNA G-Quadruplex and i-Motif Structure Formation Is Interdependent in Human Cells

AU - King, Jessica

AU - Irving, Kelly

AU - Evans, Cameron W.

AU - Chikhale, Rupesh

AU - Becker, Rouven

AU - Morris, Christopher

AU - Peña Martinez, Cristian

AU - Schofield, Peter

AU - Christ, Daniel

AU - Hurley, Laurence

AU - Waller, Zoë

AU - Swaminatha Iyer, Killugudi

AU - Smith, Nicole

PY - 2020/12/9

Y1 - 2020/12/9

N2 - Guanine- and cytosine-rich nucleic acid sequences have the potential to form secondary structures such as G-quadruplexes and i-motifs, respectively. We show that stabilization of G-quadruplexes using small molecules destabilizes the i-motifs, and vice versa, indicating these gene regulatory controllers are interdependent in human cells. This has important implications as these structures are predominately considered as isolated structural targets for therapy, but their interdependency highlights the interplay of both structures as an important gene regulatory switch.

AB - Guanine- and cytosine-rich nucleic acid sequences have the potential to form secondary structures such as G-quadruplexes and i-motifs, respectively. We show that stabilization of G-quadruplexes using small molecules destabilizes the i-motifs, and vice versa, indicating these gene regulatory controllers are interdependent in human cells. This has important implications as these structures are predominately considered as isolated structural targets for therapy, but their interdependency highlights the interplay of both structures as an important gene regulatory switch.

U2 - 10.1021/jacs.0c11708

DO - 10.1021/jacs.0c11708

M3 - Article

C2 - 33253551

SN - 0002-7863

VL - 142

SP - 20600

EP - 20604

JO - Journal of the Amercian Chemical Society

JF - Journal of the Amercian Chemical Society

IS - 49

ER -

DNA G-Quadruplex and i-Motif Structure Formation Is Interdependent in Human Cells

Abstract

Access to Document

Fingerprint

Research output

Characterisation of endogenous i-motif and G-quadruplex DNA structures in human chromatin and their relationship with cancer-associated genomic landscapes

Cite this